Mycoparasitism illuminated by genome and transcriptome sequencing of Coniothyrium minitans , an important biocontrol fungus of the plant pathogen Sclerotinia sclerotiorum

Microbial Genomics
Huizhang ZhaoYanping Fu

Abstract

Coniothyrium minitans is a mycoparasite of the notorious plant pathogen Sclerotinia sclerotiorum. To further understand the parasitism of C. minitans, we assembled and analysed its genome and performed transcriptome analyses. The genome of C. minitans strain ZS-1 was assembled into 350 scaffolds and had a size of 39.8 Mb. A total of 11 437 predicted genes and proteins were annotated, and 30.8 % of the blast hits matched proteins encoded by another member of the Pleosporales, Paraphaeosphaeria sporulosa, a worldwide soilborne fungus with biocontrol ability. The transcriptome of strain ZS-1 during the early interaction with S. sclerotiorum at 0, 4 and 12 h was analysed. The detected expressed genes were involved in responses to host defenses, including cell-wall-degrading enzymes, transporters, secretory proteins and secondary metabolite productions. Seventeen differentially expressed genes (DEGs) of fungal cell-wall-degrading enzymes (FCWDs) were up-regulated during parasitism, with only one down-regulated. Most of the monocarboxylate transporter genes of the major facilitator superfamily and all the detected ABC transporters, especially the heavy metal transporters, were significantly up-regulated. Approximately 8 % of the 11 4...Continue Reading

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Datasets Mentioned

BETA
VFEO00000000
SRR9290694
SRR9290695
SRR9290696
SRR9988701
SRR9988715

Methods Mentioned

BETA
RNA-seq
2500 sequencing
reverse transcription PCR
PCR
PCA
one hybrid

Software Mentioned

WebApollo
Cufflinks
Scaffold
GapCloser
TopHat
SOAPdenovo2
GeneMark
Diamond
EVidenceModeler
GeneModel

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