Mycoplasma bovis antibody dynamics in naturally exposed dairy calves according to two diagnostic tests

BMC Veterinary Research
Mette B PetersenLiza Rosenbaum Nielsen

Abstract

Inexpensive and convenient diagnostic tests for use in clinical work and for the surveillance of infection with Mycoplasma bovis are in demand. The objective of this longitudinal field study was to gain knowledge about the dynamics of antibodies against M. bovis in sera from naturally exposed calves with and without different clinical signs, measured by two different ELISA tests. A total of 83 calves were subject to between one and five blood samples and clinical examinations using a standard protocol during five herd visits to each of four outbreak dairy herds. The blood samples were analysed for the presence of antibodies against M. bovis using the commercial IgG ELISA test BioX K302 (BioX) and an in-house indirect IgG ELISA test (MilA ELISA). Linear mixed models were used to describe and compare the antibody dynamics as measured by the two tests in relation to the disease status and age of the animals. The BioX ELISA response was below the recommended cut-off (37 ODC%) for the entire study period in many of the calves. The estimated mean ODC% increased slowly but did not reach the recommended individual animal cut-off in three of the four herds. The highest estimated ODC% was not reached until the calf was 110-130 days old. ...Continue Reading

References

Jan 29, 2009·The Veterinary Clinics of North America. Food Animal Practice·Fiona P Maunsell, G Arthur Donovan
Dec 18, 2013·Clinical and Vaccine Immunology : CVI·Nadeeka K WawegamaPhilip F Markham

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Methods Mentioned

BETA
PCR
ELISA
ELISAs

Software Mentioned

R
lme4
R Core

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