Mycoplasma bovis isolates from dairy calves in Japan have less susceptibility than a reference strain to all approved macrolides associated with a point mutation (G748A) combined with multiple species-specific nucleotide alterations in 23S rRNA

Microbiology and Immunology
Toyotaka SatoYutaka Tamura

Abstract

Erythromycin, tylosin and tilmicosin are approved for use in cattle in Japan, the latter two being used to treat Mycoplasma bovis infection. In this study, 58 M. bovis isolates obtained from Japanese dairy calves all exhibited reduced susceptibility to these macrolides, this widespread reduced susceptibility being attributable to a few dominant lineages. All 58 isolates contained the G748A variant in both the rrl3 and rrl4 alleles of 23S rRNA, whereas a reference strain (PG45) did not. G748 localizes in the central loop of domain II (from C744 to A753) of 23S rRNA, which participates in binding to mycinose, a sugar residue present in both tylosin and tilmicosin. A number of in vitro-selected mutants derived from M. bovis PG45 showed reduced susceptibility to tylosin and tilmicosin and contained a nucleotide insertion within the central loop of domain II of rrl3 (U747-G748Ins_CU/GU or A743-U744Ins_UA), suggesting that mutations around G748 confer this reduced susceptibility phenotype. However, other Mycoplasma species containing G748A were susceptible to tylosin and tilmicosin. Sequence comparison with Escherichia coli revealed that M. bovis PG45 and isolates harbored five nucleotide alterations (U744C, G745A, U746C, A752C and A...Continue Reading

References

Oct 1, 1996·International Journal of Systematic Bacteriology·B PetterssonK E Johansson
Dec 3, 1999·Antimicrobial Agents and Chemotherapy·M C RobertsH Seppala
Oct 24, 2000·International Journal of Medical Microbiology : IJMM·R RosengartenJ Spergser
Sep 22, 2001·The Journal of Antimicrobial Chemotherapy·S Douthwaite, W S Champney
Feb 19, 2002·Antimicrobial Agents and Chemotherapy·S RaherisonC M Bébéar
Nov 6, 2002·Proceedings of the National Academy of Sciences of the United States of America·Mingfu Liu, Stephen Douthwaite
Sep 25, 2004·Antimicrobial Agents and Chemotherapy·Guy W NovotnyStephen Douthwaite
Oct 27, 2004·Antimicrobial Agents and Chemotherapy·J ViccaF Haesebrouck
Nov 25, 2004·Antimicrobial Agents and Chemotherapy·Mayumi MatsuokaTsuguo Sasaki
Sep 6, 2005·The Journal of Veterinary Medical Science·Hideki KobayashiKoshi Yamamoto
Nov 30, 2005·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Ricardo F RosenbuschLorraine J Hoffman
Mar 29, 2006·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Mihai I GageaJeff L Caswell
Jul 20, 2007·Antimicrobial Agents and Chemotherapy·N T AntunesJ B Poveda
May 30, 2009·Bioinformatics·Michiaki HamadaKiyoshi Asai
Aug 17, 2010·The Journal of Veterinary Medical Science·Ryoko UemuraHiroshi Nagatomo
Dec 8, 2010·Infection and Immunity·Kim S WiseBarbara A Methé
Sep 17, 2011·The Veterinary Record·H HiguchiH Nagahata
Dec 21, 2011·Antimicrobial Agents and Chemotherapy·Taradon LuangtongkumQijing Zhang
Nov 23, 2013·Animal Science Journal = Nihon Chikusan Gakkaihō·Kazuhiro KawaiToshio Oshida
Jan 8, 2014·Veterinary Microbiology·Uri LernerInna Lysnyansky
May 21, 2016·Frontiers in Microbiology·Inna Lysnyansky, Roger D Ayling

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.