PMID: 8454185Jan 15, 1993Paper

Mycoplasma cells stimulate in vitro activation of plasminogen by purified tissue-type plasminogen activator

FEMS Microbiology Letters
M TarshisM Mayer

Abstract

In an in vitro direct assay with tissue-type plasminogen activator (tPA), plasminogen and the chromogenic substrate S-2251, the ability of Mycoplasma fermentans KL4 to stimulate tPA-mediated activation of plasminogen to plasmin was studied. Mycoplasma cells markedly enhanced the activation of plasminogen by tPA in a concentration-, temperature- and pH-dependent manner. Nonidet P-40 (0.01%), sonication, and freezing and thawing of the cells substantially increased the stimulatory effect of mycoplasma on tPA activity. In contrast, the activation of plasminogen by urokinase was refractory to mycoplasma cells. The mycoplasma-mediated stimulation of tPA activity was prevented by epsilon-aminocaproic acid (EACA), a lysine analogue known to block lysine-binding sites (LBS) in plasminogen and tPA. Among several Mycoplasma fermentans strains tested, incognitus strain demonstrated the highest stimulation activity. These results suggest that mycoplasma cells interact with LBS in tPA and plasminogen to enhance plasminogen activation.

References

Feb 1, 1977·Journal of Bacteriology·S RottemM F Barile
May 1, 1978·FEBS Letters·M A Tarshis, A B Kapitanov
Jan 1, 1990·Infection and Immunity·M UllbergB Wiman
Apr 2, 1991·Biochimica Et Biophysica Acta·M SalmanS Rottem
Nov 12, 1985·Biochimica Et Biophysica Acta·O Saksela
Nov 1, 1989·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·M UllbergB Wiman
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Nov 1, 1989·The American Journal of Tropical Medicine and Hygiene·S C LoR Y Wang
Nov 1, 1971·Biochemical and Biophysical Research Communications·G L Choules, W R Gray

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Citations

Mar 20, 2001·Infection and Immunity·A YavlovichS Rottem
Dec 18, 2001·FEMS Microbiology Reviews·K LähteenmäkiT K Korhonen
Feb 27, 2001·Journal of Neuroscience Research·M P Flavin, G Zhao

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