PMID: 8943749Oct 1, 1996Paper

Mycoplasma fermentans, but not M penetrans, detected by PCR assays in synovium from patients with rheumatoid arthritis and other rheumatic disorders

Journal of Clinical Pathology
T SchaeverbekeD Taylor-Robinson

Abstract

Mycoplasmas, especially Mycoplasma fermentans, were suggested more than 20 years ago as a possible cause of rheumatoid arthritis but this hypothesis was never substantiated. In view of the superior sensitivity of the polymerase chain reaction (PCR) assay over culture, the aim was to use this method to seek M fermentans and M penetrans in synovial samples from patients with various arthritides. Synovial fluid samples (n = 154) and synovial biopsy specimens (n = 20) from 133 patients with various rheumatic disorders were stored at -80 degrees C for between one and 40 months. Aliquots (500 microliters) of the synovial fluid samples were centrifuged and the deposit, and also the synovial biopsy specimens (approximately 1 g) were placed in lysis buffer with proteinase K for DNA extraction. The DNA was tested by using a semi-nested PCR assay for M fermentans and a single-round PCR for M penetrans. M fermentans was detected in the joints of eight (21%) of 38 patients with rheumatoid arthritis, two (20%) of 10 patients with spondyloarthropathy with peripheral arthritis, one (20%) of five patients with psoriatic arthritis, and four (13%) of 31 patients with unclassified arthritis. M fermentans was not found in the joints of the seven pa...Continue Reading

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Citations

Jan 9, 2007·Clinical Rheumatology·Taha Rashid, Alan Ebringer
Oct 6, 2000·Microbiology and Immunology·K MatsudaN Yamamoto
Oct 15, 2005·Clinical Microbiology Reviews·Ken B WaitesRobert L Schelonka
Oct 14, 2004·Annals of the Rheumatic Diseases·S M CartyA J Silman
Feb 15, 2001·Annals of the Rheumatic Diseases·D Taylor-Robinson, A Keat
Feb 6, 2010·The Journal of Veterinary Medical Science·Masatoshi FujiharaRyô Harasawa
Mar 17, 2010·Journal of Bioscience and Bioengineering·Noriko IshidaKozo Asano
Nov 23, 2006·Infectious Disease Clinics of North America·Raquel Cuchacovich
Mar 15, 2003·Rheumatic Diseases Clinics of North America·Raquel CuchacovichAna Maria Santos
Mar 1, 2008·Biochemical and Biophysical Research Communications·Yutaka KawahitoKazuhiro Matsuda
Sep 26, 2007·Biologicals : Journal of the International Association of Biological Standardization·Baharak AfsharRoger J Miles
Oct 17, 2006·Journal of Clinical Rheumatology : Practical Reports on Rheumatic & Musculoskeletal Diseases·Eugene EskowEli Mordechai
Mar 3, 2010·Immunological Reviews·Beatrix Bartok, Gary S Firestein
Aug 24, 2010·Journal of Cutaneous Pathology·Belinda H TanPaul K Shitabata
Oct 18, 2007·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Lisa M Ganley-LealSalvatore Frasca
Aug 15, 2014·Molecular Medicine Reports·Kunpeng WangHongqiang Li
Jan 7, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sarah L JongbloedJames M Brewer
Jun 22, 1999·Infection and Immunity·L ReyesJ K Davis
Dec 5, 1998·Microbiology and Molecular Biology Reviews : MMBR·S RazinY Naot

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