Mycoplasma hyorhinis-encoded cytidine deaminase efficiently inactivates cytosine-based anticancer drugs

FEBS Open Bio
J Vande VoordeJan Balzarini

Abstract

Mycoplasmas may colonize tumor tissue in patients. The cytostatic activity of gemcitabine was dramatically decreased in Mycoplasma hyorhinis-infected tumor cell cultures compared with non-infected tumor cell cultures. This mycoplasma-driven drug deamination could be prevented by exogenous administration of the cytidine deaminase (CDA) inhibitor tetrahydrouridine, but also by the natural nucleosides or by a purine nucleoside phosphorylase inhibitor. The M. hyorhinis-encoded CDAHyor gene was cloned, expressed as a recombinant protein and purified. CDAHyor was found to be more catalytically active than its human equivalent and efficiently deaminates (inactivates) cytosine-based anticancer drugs. CDAHyor expression at the tumor site may result in selective drug inactivation and suboptimal therapeutic efficiency.

References

Oct 1, 1973·Journal of Biochemistry·H Hosono, S Kuno
Sep 1, 1996·Protein Expression and Purification·S VincenzettiA Vita
Dec 5, 1998·Microbiology and Molecular Biology Reviews : MMBR·S RazinY Naot
Jan 31, 2002·World Journal of Gastroenterology : WJG·S HuangC C Shou
Jun 16, 2004·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Mustafa PehlivanSacide Pehlivan
Jul 1, 2010·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Takahiro YoshidaTakuma Sasaki
Apr 8, 2011·Oncotarget·Yulia A BarykovaAndrei V Gudkov
Oct 18, 2011·Journal of Cardiothoracic Surgery·Panagiotis ApostolouGregory Giamouzis
Sep 25, 2012·International Urology and Nephrology·Sakip M ErturhanMetin Karakök
Jun 1, 2013·Nature Reviews. Drug Discovery·Lars Petter JordheimCharles Dumontet
Jun 14, 2014·The Journal of Antimicrobial Chemotherapy·Johan Vande VoordeSandra Liekens

❮ Previous
Next ❯

Citations

Jan 31, 2017·Current Opinion in Infectious Diseases·Hans Helmut NillerJanos Minarovits
Oct 3, 2018·Expert Opinion on Drug Metabolism & Toxicology·Ramy K AzizMarwa T ElRakaiby
Feb 15, 2020·Investigative and Clinical Urology·Sybil ShaVladimir Mouraviev
May 22, 2021·Organic & Biomolecular Chemistry·Paul T LudfordYitzhak Tor

❮ Previous
Next ❯

Methods Mentioned

BETA
deamination
xenografts

Software Mentioned

GraphPad Prism5

Related Concepts

Related Feeds

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.