Mycoplasma iowae species-specific DNA probe

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
S Zhao, R Yamamoto

Abstract

A Mycoplasma iowae (MI) species-specific DNA probe (designated pMI-2) of 6.0 kbp (kilobase pairs) was isolated from an MI strain I-695 genomic library prepared in plasmid pUC8 and Escherichia coli strain JM83. When labeled with [32]P by nick translation, the probe hybridized in dot blot assays with 6 reference strains and 8 field isolates of MI but not with 16 other known species of avian mycoplasmas. The pMI-2 probe detected a minimum of 1.5 ng of MI strain I-695 chromosomal DNA. Under identical conditions of hybridization, the probe did not hybridize with a high concentration (200 ng) of M. gallisepticum or M. synoviae chromosomal DNA.

References

Apr 1, 1989·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·S H Zhao, R Yamamoto
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Citations

May 1, 1993·Veterinary Microbiology·S Zhao, R Yamamoto
Jul 1, 1993·Veterinary Microbiology·S Zhao, R Yamamoto
Jun 1, 1996·Avian Pathology : Journal of the W.V.P.A·A R Al-Ankari, J M Bradbury

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