Mycoplasma pneumoniae and Legionella pneumophila in community-acquired lower respiratory tract infections among hospitalized children: diagnosis by real time PCR

Scandinavian Journal of Infectious Diseases
Helen C MaltezouDidier Raoult

Abstract

Mycoplasma pneumoniae and Legionella pneumophila are increasingly recognized as important agents of community-acquired lower respiratory tract infections (LRTI). Mycoplasma pneumoniae has been also recognized as a cause of nosocomial infections. The aim of this study was to investigate the role of real time polymerase chain reaction (PCR) for the rapid diagnosis of these infections among hospitalized children with community-acquired LRTI. During 2001, 65 children were prospectively studied. Microbiological investigation consisted of capillary PCR with a LightCycler for M. pneumoniae and L. pneumophila in induced sputum or throat swab specimens, IgM enzyme immunoassay for M. pneumoniae and immunofluorescence for L. pneumophila in paired sera. Serology testing showed acute M. pneumoniae infection in 18 (27.5%) patients and L. pneumophila in 1 (1.5%). M. pneumoniae was also detected in sputum specimen by capillary PCR in 9 (50%) serologically diagnosed cases, including 4 (22%) with non-diagnostic IgM levels in the acute phase. Capillary PCR and IgM enzyme immunoassay diagnosed together 15 (83%) M. pneumoniae cases in the acute phase. It is concluded that M. pneumoniae is an important cause of LRTI necessitating hospitalization amo...Continue Reading

References

Apr 1, 1985·Statistics in Medicine·O Miettinen, M Nurminen
May 7, 2002·The Journal of Infectious Diseases·Danuta M SkowronskiScott A Halperin
May 15, 2003·Vaccine·Marie Van der WielenClaire-Anne Siegrist

❮ Previous
Next ❯

Citations

Jun 7, 2013·Journal of Clinical Microbiology·Sudhir SinghDidier Raoult
Feb 9, 2006·Archives of Disease in Childhood·G KarevoldK J Kvaerner
Dec 6, 2005·Journal of Biomedical Science·Wen-Bin LiuCheng-Chao Shou
Mar 29, 2014·BioMed Research International·Giovanni GabuttiGiorgio Zoppi
Nov 5, 2008·Expert Review of Vaccines·Wayde M Weston, Nicola P Klein
Mar 4, 2010·The Annals of Pharmacotherapy·Tracy L Johns, George E Hutter
Aug 30, 2008·FEMS Microbiology Reviews·Thomas Prescott AtkinsonKen B Waites

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Related Papers

Pediatrics International : Official Journal of the Japan Pediatric Society
Fujio KakuyaHiroki Kano
The Brazilian Journal of Infectious Diseases : an Official Publication of the Brazilian Society of Infectious Diseases
Nahal HadiNima Rezaei
Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America
Jan Jelrik OosterheertMarc J M Bonten
© 2021 Meta ULC. All rights reserved