PMID: 9423868Jan 10, 1998Paper

Mycoplasma pulmonis inhibits electrogenic ion transport across murine tracheal epithelial cell monolayers

Infection and Immunity
L C LambertR J Bridges

Abstract

Murine chronic respiratory disease is characterized by persistent colonization of tracheal and bronchial epithelial cell surfaces by Mycoplasma pulmonis, submucosal and intraluminal immune and inflammatory cells, and altered airway activity. To determine the direct effect of M. pulmonis upon transepithelial ion transport in the absence of immune and inflammatory cell responses, primary mouse tracheal epithelial cell monolayers (MTEs) were apically infected and assayed in Ussing chambers. M. pulmonis-infected MTEs, but not those infected with a nonmurine mycoplasma, demonstrated reductions in amiloride-sensitive Na+ absorption, cyclic AMP, and cholinergic-stimulated Cl- secretion and transepithelial resistance. These effects were shown to require interaction of viable organisms with the apical surface of the monolayer and to be dependent upon organism number and duration of infection. Altered transport due to M. pulmonis was not merely a result of epithelial cell death as evidenced by the following: (i) active transport of Na+ and Cl-, albeit at reduced rates; (ii) normal cell morphology, including intact tight junctions, as demonstrated by electron microscopy; (iii) maintenance of a mean transepithelial resistance of 440 omega/...Continue Reading

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Citations

Sep 30, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·André DagenaisYves Berthiaume
Oct 29, 2005·American Journal of Respiratory and Critical Care Medicine·Judy M Hickman-DavisSadis Matalon
Sep 2, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ian C DavisSadis Matalon
Feb 9, 2007·Journal of Virology·Karl KunzelmannDavid I Cook
Jan 27, 2012·American Journal of Physiology. Cell Physiology·Yawar J QadriCatherine M Fuller
Apr 28, 2004·The American Journal of Pathology·Michelle M CloutierRoger S Thrall
Jul 17, 1999·Pharmaceutical Science & Technology Today·A L Daugherty, R J Mrsny
Jul 13, 2000·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·P C YangZ D Tao

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