"Mycoplasmal antigen modulation," a novel surface variation suggested for a lipoprotein specifically localized on Mycoplasma mobile.

Current Microbiology
Heng Ning WuMakoto Miyata

Abstract

Mycoplasma mobile, a pathogen of freshwater fish, glides easily across surfaces, colonizes on the fish gill, and causes necrosis. The cell surface is differentiated into three parts: the head, neck, and body. Mobile variable surface proteins (Mvsps) localizing at each of these parts may be involved in surface variation including phase variation and antigenic variation, although no proof exists. In this study, we examined this possibility by focusing on MvspI, the largest Mvsp. Immunofluorescence microscopy showed that MvspI is expressed on the surfaces of all cells. When anti-MvspI antibody was added at concentrations over 0.8 nM, MvspI was observed to decrease over time. After 72 h of cultivation with the antibody, the fluorescence intensity and amount of MvspI decreased up to 13 and 39%, respectively, compared to those of cells grown without antibody. These changes were reversed by the removal of the antibody. Such effects were not observed when another antibody targeting other Mvsps was used, suggesting that the decrease is specific to the relationship between MvspI and the antibody. Cell growth was also inhibited by the antibody, but the decrease in MvspI could not be explained by the selective growth of MvspI-negative vari...Continue Reading

References

Dec 1, 1989·Journal of Bacteriology·W G WeisburgJ Van Etten
May 1, 1987·Journal of Bacteriology·R Rosengarten, H Kirchhoff
Sep 1, 1984·Journal of General Microbiology·H Kirchhoff, R Rosengarten
Dec 5, 1998·Microbiology and Molecular Biology Reviews : MMBR·S RazinY Naot
Nov 26, 1999·Biochimie·M Miyata, S Seto
Apr 27, 2000·Journal of Bacteriology·X ShenK Dybvig
May 23, 2001·Nucleic Acids Research·I ChambaudA Blanchard
Mar 13, 2002·Journal of Bacteriology·Makoto MiyataHoward C Berg
Nov 8, 2002·Molecular Microbiology·Ramakrishnan SitaramanKevin Dybvig
Jul 20, 2004·Clinical Microbiology Reviews·Marjan W van der Woude, Andreas J Bäumler
Aug 4, 2004·Genome Research·Jacob D JaffeGeorge M Church
Sep 20, 2006·Journal of Molecular Microbiology and Biotechnology·Makoto Miyata, Hiroshi Ogaki
May 22, 2007·PLoS Genetics·Pascal Sirand-PugnetChristine Citti
Aug 10, 2007·Journal of Molecular Evolution·Kenro Oshima, Hiromi Nishida
Nov 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·Daisuke Nakane, Makoto Miyata
Dec 18, 2007·Trends in Microbiology·Makoto Miyata
Nov 17, 2009·Journal of Bacteriology·Takahiro NonakaMakoto Miyata
Jun 11, 2010·Annual Review of Microbiology·Makoto Miyata
Jul 17, 2010·Future Microbiology·Christine CittiEric Baranowski

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