Mycosubtilin overproduction by Bacillus subtilis BBG100 enhances the organism's antagonistic and biocontrol activities

Applied and Environmental Microbiology
Valérie LeclèrePhilippe Jacques

Abstract

A Bacillus subtilis derivative was obtained from strain ATCC 6633 by replacement of the native promoter of the mycosubtilin operon by a constitutive promoter originating from the replication gene repU of the Staphylococcus aureus plasmid pUB110. The recombinant strain, designated BBG100, produced up to 15-fold more mycosubtilin than the wild type produced. The overproducing phenotype was related to enhancement of the antagonistic activities against several yeasts and pathogenic fungi. Hemolytic activities were also clearly increased in the modified strain. Mass spectrometry analyses of enriched mycosubtilin extracts showed similar patterns of lipopeptides for BBG100 and the wild type. Interestingly, these analyses also revealed a new form of mycosubtilin which was more easily detected in the BBG100 sample. When tested for its biocontrol potential, wild-type strain ATCC 6633 was almost ineffective for reducing a Pythium infection of tomato seedlings. However, treatment of seeds with the BBG100 overproducing strain resulted in a marked increase in the germination rate of seeds. This protective effect afforded by mycosubtilin overproduction was also visualized by the significantly greater fresh weight of emerging seedlings treated...Continue Reading

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Citations

Sep 17, 2008·Archives of Microbiology·Marlène Chollet-ImbertPhilippe Jacques
Apr 25, 2007·Applied Microbiology and Biotechnology·Shinji MizumotoMakoto Shoda
Jul 14, 2011·Applied Microbiology and Biotechnology·Ahmed AbderrahmaniPhilippe Jacques
Jun 9, 2012·Applied Microbiology and Biotechnology·Max BéchetPhilippe Jacques
Nov 13, 2012·Current Microbiology·Csaba VágvölgyiLászló Manczinger
Oct 16, 2012·Applied Biochemistry and Biotechnology·O TabbeneF Limam
Apr 16, 2013·Bioresource Technology·Max BéchetPhilippe Jacques
Feb 18, 2012·Natural Product Research·S ElkahouiHartmut Laatsch
Jul 15, 2006·Molecular Plant-microbe Interactions : MPMI·Jos M RaaijmakersMaarten J D de Kock
Apr 13, 2007·Molecular Plant-microbe Interactions : MPMI·Diego RomeroAlejandro Pérez-García
Jun 7, 2007·Microbial Cell Factories·Muaaz Mutaz Al-AjlaniShahida Hasnain
Oct 1, 2013·Biocontrol Science·Caroline F Ajilogba, Olubukola O Babalola
Apr 12, 2014·Applied Microbiology and Biotechnology·Jovana DeravelPhilippe Jacques
Dec 18, 2009·International Journal of Microbiology·M LeónA F García
Jul 26, 2008·Archives of Microbiology·Augusto EtchegarayOmar Teschke
Dec 24, 2015·Frontiers in Microbiology·Liming WuRainer Borriss
Jul 17, 2015·Environmental Science and Pollution Research International·Ines MnifDhouha Ghribi
May 18, 2012·TheScientificWorldJournal·M G L Basurto-CadenaJ E Barboza-Corona
Jul 21, 2015·Peptides·Mnif Inès, Ghribi Dhouha
Apr 29, 2015·Frontiers in Plant Science·Walaa K Mousa, Manish N Raizada

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