Mycoviruses of an endophytic fungus can replicate in plant cells: evolutionary implications

Scientific Reports
L NervaM Turina

Abstract

So far there is no record of a specific virus able to infect both fungal and plant hosts in nature. However, experimental evidence shows that some plant virus RdRPs are able to perform replication in trans of genomic or DI RNAs in the yeast Saccharomyces cerevisiae. Furthermore, tobacco mosaic virus was recently shown to replicate in a filamentous ascomycetous fungus. Thus, at least experimentally, some plant viruses can infect some fungi. Endophytic fungi have been reported from many plants and several of these fungi have been shown to contain viruses. Here we tested if mycoviruses derived from a marine plant endophyte can replicate in plant cells. For this purpose, we used partially purified viral particles from isolate MUT4330 of Penicillium aurantiogriseum var. viridicatum which harbors six virus species, some having dsRNA and some positive-strand ssRNA genomes. These were transfected into three distinct plant protoplast cell systems. Time-course analysis of absolute RNA accumulation provided for the first time evidence that viruses of two species belonging to the Partitiviridae and Totiviridae families, can replicate in plant cells without evidence of host adaptation, i.e, changes in their nucleotide sequence.

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Citations

Nov 1, 2017·Proceedings of the National Academy of Sciences of the United States of America·Ida Bagus AndikaLiying Sun
Aug 8, 2018·The New Phytologist·Marilyn J Roossinck
Jul 29, 2018·Molecular Plant Pathology·Walter ChitarraGiorgio Gambino
Aug 1, 2019·FEMS Microbiology Ecology·Suvi SutelaEeva J Vainio
Oct 6, 2018·Environmental Microbiology·L NervaM Turina
Jun 12, 2019·Proceedings of the National Academy of Sciences of the United States of America·Shuang WeiLiying Sun
Jul 18, 2019·Nature Reviews. Microbiology·Pierre LefeuvreArvind Varsani
May 11, 2020·Virus Genes·Abu Bakar Siddique
Jun 24, 2019·Virus Genes·Karel Petrzik
Feb 26, 2019·Scientific Reports·Tiziana MasciaDonato Gallitelli
Feb 6, 2020·Proceedings of the National Academy of Sciences of the United States of America·Ruiling BianLiying Sun
Jul 17, 2020·Frontiers in Microbiology·Paul TelengechNobuhiro Suzuki
Nov 17, 2020·Frontiers in Microbiology·Syun-Ichi UrayamaTakuro Nunoura
Dec 5, 2020·Plant, Cell & Environment·Rouhallah Sharifi, Choong-Min Ryu
Aug 28, 2021·Microorganisms·Gabriel Quintanilha-PeixotoEric Roberto Guimarães Rocha Aguiar

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Methods Mentioned

BETA
transfect
transfection
PCR
transfections
transgenic
electrophoresis

Software Mentioned

Systat
Bio
SIGMAPLOT
Rad CFX Manager

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