MyD88 and NOS2 are essential for toll-like receptor 4-mediated survival effect in cardiomyocytes

American Journal of Physiology. Heart and Circulatory Physiology
Xinsheng ZhuWei Chao

Abstract

Innate immune system such as Toll-like receptor 4 (TLR4) represents the first line of defense against infection. In addition to its pivotal role in host immunity, recent studies have suggested that TLR4 may play a broader role in mediating tissue inflammation and cell survival in response to noninfectious injury. We and other investigators have reported that cardiac TLR4 signaling is dynamically modulated in ischemic myocardium and that activation of TLR4 confers a survival benefit in the heart and in isolated cardiomyocytes. However, the signaling pathways leading to these effects are not completely understood. Here, we investigate the role of MyD88, an adaptor protein of TLR4 signaling, and inducible nitric oxide synthase (NOS2) in mediating TLR4-induced cardiomyocyte survival in an in vitro model of apoptosis. Serum deprivation induced a significant increase in the number of apoptotic cardiomyocytes as demonstrated by transferase-mediated dUTP nick-end labeling (TUNEL) assay, nuclear morphology, DNA laddering, and DNA-histone ELISA. Lipopolysaccharide (LPS), a TLR4 agonist, activated TLR4 signaling and led to significant reduction in apoptotic cardiomyocytes and improved cellular function of surviving cardiomyocytes with enh...Continue Reading

References

Jan 1, 1991·The Journal of Clinical Investigation·A A KnowltonC S Apstein
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J M BrownJ E Repine
Nov 7, 1995·Proceedings of the National Academy of Sciences of the United States of America·V E LaubachP A Sherman
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·M BuerkeA M Lefer
Sep 26, 1997·The Journal of Biological Chemistry·J B MannickJ S Stamler
Oct 13, 1998·Journal of the American College of Cardiology·H DrexlerG Hasenfuss
Mar 6, 1999·The Journal of Biological Chemistry·L RössigS Dimmeler
Aug 3, 1999·The Journal of Clinical Investigation·S FrantzR A Kelly
Aug 6, 1999·Immunity·T KawaiS Akira
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·Y GuoR Bolli
Jan 7, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·K OhashiH Kolb
Jul 6, 2000·The EMBO Journal·A O AliprantisA Zychlinsky
Nov 18, 2000·The Journal of Biological Chemistry·S FrantzT Bourcier
Sep 12, 2001·The Journal of Cell Biology·J B MannickB Gaston
Feb 14, 2002·The Journal of Biological Chemistry·Ramunas M VabulasHermann Wagner
May 11, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Shintaro NemotoDouglas L Mann
Jun 14, 2002·The Journal of Biological Chemistry·Wei ChaoAnthony Rosenzweig
Jun 19, 2002·Circulation·S R KirchhoffA A Knowlton
Jan 14, 2003·Annual Review of Immunology·Kiyoshi TakedaShizuo Akira
May 17, 2003·The Journal of Clinical Investigation·Detlef WenckerRichard N Kitsis
Jan 1, 2004·The Journal of Biological Chemistry·Ji-Eun Kim, Steven R Tannenbaum
Mar 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·Federica del MonteRoger J Hajjar

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Citations

Jul 29, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Yan FengWei Chao
Jun 9, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Emmanuel S BuysKenneth D Bloch
Nov 18, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Wei Chao
Jun 27, 2007·BMC Physiology·Se-Chan KimGeorg Baumgarten
Feb 4, 2014·Life Sciences·Li Lin, Anne A Knowlton
Mar 22, 2016·Journal of Immunology Research·Jun Xu, Ulka Sachdev
Oct 16, 2015·Journal of Pineal Research·Frederic NduhirabandiSandrine Lecour
Oct 17, 2012·Vascular Pharmacology·Ali NaviJanice Tsui
Nov 16, 2010·Journal of Molecular and Cellular Cardiology·Veli K TopkaraDouglas L Mann
Dec 17, 2008·Free Radical Biology & Medicine·Crystal M ReynoldsClaude A Piantadosi
Jun 21, 2012·Biochemical and Biophysical Research Communications·Yuehai WangYun Zhang
Oct 23, 2012·International Reviews of Immunology·Faezeh Moghimpour BijaniNima Rezaei
Mar 10, 2011·Expert Opinion on Therapeutic Targets·Ulrich HofmannStefan Frantz
Dec 17, 2010·Journal of Translational Medicine·Yuwei ZhangWei-Ping Min
Jun 2, 2016·Clinical and Experimental Pharmacology & Physiology·Sam Man LeeDavid A Saint
Sep 29, 2017·Cardiovascular Drugs and Therapy·Karam F AyoubJawahar L Mehta
Jan 7, 2014·Journal of the American Heart Association·Chan ChenWei Chao
Jan 10, 2012·Circulation Research·Matthew Coggins, Anthony Rosenzweig
Feb 1, 2011·Anesthesiology·Tobias Eckle, Holger K Eltzschig
Jul 27, 2007·Nature Clinical Practice. Cardiovascular Medicine·Stefan FrantzJohann Bauersachs
Oct 2, 2008·Nature Reviews. Drug Discovery·Rahul Kakkar, Richard T Lee
May 28, 2019·American Journal of Physiology. Heart and Circulatory Physiology·Weiang YanSanjiv Dhingra
Jul 7, 2017·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Li LiuJena J Steinle
Mar 29, 2018·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Yuwei ZhangPing Cheng
Apr 15, 2020·Diabetes Therapy : Research, Treatment and Education of Diabetes and Related Disorders·Yuwei ZhangWeiping Min
Jul 27, 2021·Frontiers in Cellular and Infection Microbiology·Mohammad AqdasJaved Naim Agrewala
Aug 10, 2021·Journal of Molecular and Cellular Cardiology·Abraham L Bayer, Pilar Alcaide
Aug 10, 2021·Frontiers in Cardiovascular Medicine·Tianyi ZhangZhipeng Cao

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