MyD88 Deficiency Protects Against Dry Eye-Induced Damage

Investigative Ophthalmology & Visual Science
Rose Y ReinsRachel L Redfern

Abstract

Dry eye disease (DED) is a multifactorial disease associated with ocular surface inflammation. Toll-like receptors (TLRs) are integral in the initiation of inflammatory signaling. Therefore, we evaluated the effect of TLR-deficiency on dry eye-related ocular surface damage and inflammation using a mouse model of experimental dry eye (EDE). C57BL/6 wild-type (WT), MyD88-/-, and IL-1R-/- mice were exposed to EDE conditions for 5 days. Tear production was measured by phenol red thread test and ocular surface damage assessed with fluorescein staining. Corneal homogenates were obtained for matrix metalloproteinase (MMP) and cytokine expression analysis by Luminex assay and quantitative PCR. In addition, whole eyes and eyelids were dissected and goblet cells and Meibomian glands were imaged, respectively. Following 5 days of EDE, WT mice had extensive ocular surface staining, while MyD88-/- mice had no increased staining above non-EDE conditions. Similarly, MyD88-/- mice did not have increased corneal MMP-2, 3, or 8 concentrations, as seen with WT mice. MyD88-deficiency also resulted in decreased corneal cytokine levels. In addition, MyD88-/- mice had significantly lower conjunctival goblet cell counts compared with both WT (EDE) and...Continue Reading

References

Nov 9, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·O TakeuchiS Akira
Jan 14, 2003·Annual Review of Immunology·Kiyoshi TakedaShizuo Akira
Apr 27, 2004·Experimental Eye Research·Ilene K Gipson
Nov 24, 2004·Investigative Ophthalmology & Visual Science·De-Quan LiStephen C Pflugfelder
Jan 6, 2005·The American Journal of Pathology·Stephen C PflugfelderM Elizabeth Fini
Jan 27, 2005·Investigative Ophthalmology & Visual Science·Angela C JohnsonEric Pearlman
Jan 18, 2006·Cell Death and Differentiation·T Kawai, S Akira
Aug 1, 2006·Investigative Ophthalmology & Visual Science·Rosa M CorralesStephen C Pflugfelder
Aug 11, 2006·The Ocular Surface·Patricia BuchholzFrancisco C Figueiredo
Oct 27, 2006·Investigative Ophthalmology & Visual Science·Xi HuangLinda D Hazlett
Jan 12, 2007·The Ocular Surface·Michael E Stern, Stephen C Pflugfelder
Jan 12, 2007·The Ocular Surface·De-Quan Li, Stephen C Pflugfelder
Sep 5, 2008·Current Opinion in Allergy and Clinical Immunology·Flavio Mantelli, Pablo Argüeso
Sep 11, 2008·The Ocular Surface·Eric PearlmanPaul G McMenamin
Feb 27, 2009·Mucosal Immunology·C S De PaivaS C Pflugfelder
Mar 4, 2009·Investigative Ophthalmology & Visual Science·Suksri ChotikavanichStephen C Pflugfelder
Mar 11, 2009·Arquivos brasileiros de oftalmologia·C S de Paiva, S C Pflugfelder
Jun 18, 2009·Mucosal Immunology·S K Chauhan, R Dana
Mar 30, 2010·Experimental Eye Research·Rachel L Redfern, Alison M McDermott
May 21, 2010·Mucosal Immunology·M E SternS C Pflugfelder
Sep 10, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yan SunEric Pearlman
Sep 2, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chris S SchaumburgMichael E Stern
Jan 11, 2012·Archives of Ophthalmology·William StevensonReza Dana
May 1, 2012·American Journal of Ophthalmology·Andre OkanoboReza Dana
Jul 14, 2012·Investigative Ophthalmology & Visual Science·Hyun Soo LeeReza Dana
Feb 2, 2013·Investigative Ophthalmology & Visual Science·Rachel L RedfernAlison M McDermott
May 15, 2013·The American Journal of Pathology·Christina K MarkoIlene K Gipson
Sep 21, 2013·Investigative Ophthalmology & Visual Science·Yong Woo JiHyung Keun Lee
Nov 21, 2013·Investigative Ophthalmology & Visual Science·Arantxa AceraJuan A Duran
Jul 19, 2014·Investigative Ophthalmology & Visual Science·Trinka VijmasiNancy A McNamara
Oct 14, 2014·Frontiers in Immunology·Takumi Kawasaki, Taro Kawai
Mar 31, 2015·Experimental Eye Research·Rachel L RedfernAlison M McDermott
Apr 8, 2015·Current Protocols in Immunology·Kiyoshi Takeda, Shizuo Akira
Dec 8, 2015·Investigative Ophthalmology & Visual Science·Rose Yvonne ReinsAlison Marie McDermott
Apr 20, 2016·Progress in Retinal and Eye Research·Ilene K Gipson
Aug 2, 2016·Korean Journal of Ophthalmology : KJO·Wungrak ChoiHyung Keun Lee
Aug 11, 2017·PloS One·Rose Y ReinsRachel L Redfern

❮ Previous
Next ❯

Citations

Jan 30, 2019·Investigative Ophthalmology & Visual Science·Xiaozhao ZhangLan Gong
Oct 17, 2019·Investigative Ophthalmology & Visual Science·Changjun WangCintia S de Paiva
Feb 24, 2021·The Ocular Surface·Jeremias G Galletti, Cintia S de Paiva
Apr 2, 2021·Orbit·Edward J WladisAlejandro P Adam
May 29, 2021·Experimental Eye Research·Nikhil G MenonTannin A Schmidt
Jun 17, 2021·Investigative Ophthalmology & Visual Science·Zhenying ShangJoyce Tombran-Tink

❮ Previous
Next ❯

Methods Mentioned

BETA
Assay
PCR

Software Mentioned

Prism
ImageJ
MATLAB
GraphPad

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Investigative Ophthalmology & Visual Science
Carolina LemaRachel L Redfern
Investigative Ophthalmology & Visual Science
Yang WuZuguo Liu
© 2022 Meta ULC. All rights reserved