Myelin basic protein mediates extracellular signals that regulate microtubule stability in oligodendrocyte membrane sheets

Journal of Neuroscience Research
C A DyerS Billings-Gagliardi

Abstract

Treatment of cultured oligodendrocytes with a monoclonal antibody to galactocerebroside (GalC) triggers a cascade of events including the redistribution of membrane surface GalC over internal domains of MBP and loss of microtubular structures within the sheets (Dyer and Benjamins: J Neurosci 8:4307-4318, 1988; Dyer and Benjamins: J Neurosci Res 24:212-221, 1989). In this report, wild type and myelin basic protein (MBP)-deficient shiverer oligodendrocytes were used to study the possible relationships between these events, and specifically to determine if MBP mediates signals which destabilize microtubular assemblies in cultured oligodendrocytes. We now show that MBP and GalC, which are both initially Triton X-100 soluble, become Triton X-100 insoluble following anti-GalC binding and anti-GalC:GalC complex redistribution, suggesting that the surface anti-GalC: GalC complexes become associated with cytoplasmic MBP. Mediation of the signaling event by MBP is further demonstrated by 1) a decreased phosphorylation of MBP in wild type oligodendrocytes after antibody binding, and 2) the absence of responses, such as GalC redistribution and microtubule loss, in MBP-deficient shiverer oligodendrocytes treated with anti-GalC. Continuous a...Continue Reading

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Citations

Feb 5, 2000·Journal of Neuroscience Research·C Richter-Landsberg
Jun 1, 1995·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·B Soliven, S Szuchet
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Jan 19, 2016·Brain Research·Maria Elena de Bellard
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Sep 30, 2008·European Journal of Oral Sciences·Hyun-Jin KimSun-Hun Kim
Feb 13, 2013·Journal of Neurochemistry·George Harauz, Joan M Boggs
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