Myelinogenic potential of an immortalized oligodendrocyte cell line

Journal of Neuroscience Research
S L NewmanJ W Bigbee

Abstract

The myelinogenic potential of an oligodendrocyte cell line (N20.1) immortalized by transformation with a temperature-sensitive retrovirus (Verity et al., J Neurochem 60:577-587, 1993) has been evaluated in a co-culture system utilizing dorsal root ganglion neurons. When N20.1 cells were placed in co-culture with dorsal root ganglion neurons at 39 degrees C, the temperature at which TAg expression is decreased relative to that in cells maintained at 34 degrees C, there was a dramatic decrease in the N20.1 proliferation rate compared to cells maintained in the absence of neurons at either temperature. This decrease in proliferation was observed within 3 days of co-culture and appeared to precede a further decrease in TAg expression that occurred with time in response to the neurons. In co-cultures the immunoreactivity of N20.1 cells for galactocerebroside increased with time, and the cells appeared to establish contact with neurites and initiate formation of membranous sheets. When the duration of co-culture was extended to 52 days, myelin-like figures were noted by electron microscopy. Thus, the extent of N20.1 differentiation is dependent on the presence of neurons and the duration of co-culture. This culture system represents ...Continue Reading

References

Sep 1, 1992·Journal of Neuroscience Research·R S Vick, G H DeVries
Apr 1, 1990·Journal of Neuroscience Research·R S VickG H DeVries
Jan 1, 1991·Annals of the New York Academy of Sciences·R P Skoff, P E Knapp
Jan 1, 1991·Annals of the New York Academy of Sciences·P E Knapp, R P Skoff
Sep 1, 1990·The Journal of Cell Biology·R ArmstrongM Dubois-Dalcq
Apr 1, 1983·Developmental Biology·J H CarsonE Barbarese
Oct 1, 1993·Journal of Neuroscience Research·R Hardy, R Reynolds
Jun 1, 1993·Trends in Cell Biology·S E PfeifferR Bansal

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Citations

Apr 1, 1996·Neuropathology and Applied Neurobiology·I D Duncan
Dec 4, 2010·Neurochemical Research·George H De Vries, Anne I Boullerne
Jun 20, 2003·Journal of Neuroscience Research·Diane M Studzinski, Joyce A Benjamins
Feb 26, 1999·Journal of Neurochemistry·A I BoullerneJ A Benjamins

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