Myeloid Dendritic Cells Induce HIV Latency in Proliferating CD4+ T Cells

The Journal of Immunology : Official Journal of the American Association of Immunologists
Nitasha KumarPaul U Cameron

Abstract

HIV latency occurs predominantly in long-lived resting CD4+ T cells; however, latent infection also occurs in T cell subsets, including proliferating CD4+ T cells. We compared the establishment and maintenance of latent infection in nonproliferating and proliferating human CD4+ T cells cocultured with syngeneic myeloid dendritic cells (mDC). Resting CD4+ T cells were labeled with the proliferation dye eFluor 670 and cultured alone or with mDC, plasmacytoid dendritic cells, or monocytes in the presence of staphylococcal enterotoxin B (SEB). Cells were cultured for 24 h and infected with CCR5-tropic enhanced GFP (EGFP) reporter HIV. Five days postinfection, nonproductively infected EGFP- CD4+ T cells that were either nonproliferating (eFluor 670hi) or proliferating (eFluor 670lo) were sorted and cultured for an additional 7 d (day 12) with IL-7 and antiretrovirals. At day 5 postinfection, sorted, nonproductively infected T cells were stimulated with anti-CD3/CD28, and induced expression of EGFP was measured to determine the frequency of latent infection. Integrated HIV in these cells was confirmed using quantitative PCR. By these criteria, latent infection was detected at day 5 and 12 in proliferating T cells cocultured with mDC ...Continue Reading

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Citations

May 23, 2019·PLoS Pathogens·Matthew GagneAndrew J Henderson
Jul 5, 2019·Journal of Virology·Paul LopezThomas T Murooka
Feb 29, 2020·PLoS Pathogens·Renée M Van der SluisPaul U Cameron
Jun 4, 2019·Frontiers in Immunology·Jake William RhodesStuart Grant Turville
May 2, 2020·Viruses·Maeva Dupont, Quentin James Sattentau
Jan 8, 2020·Viruses·Jan KristoffRobbie B Mailliard
Jan 29, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Renée M Van der SluisSharon R Lewin

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