Myeloid progenitors: a radiation countermeasure that is effective when initiated days after irradiation

Radiation Research
Vijay K SinghAnna K Sedello

Abstract

The aim of this study was to elucidate the potential of mouse myeloid progenitor cells (mMPC) to mitigate lethal doses of (60)Co γ radiation and X rays in various strains of mice. Different cell doses of pooled allogeneic mMPC generated ex vivo from AKR, C57Bl/6, and FVB mice were transfused intravenously into haplotype-mismatched recipient Balb/c or CD2F1 mice at various times after irradiation to assess their effect on 30-day survival. Our results show that cryopreserved allogeneic mMPC significantly improve survival in both strains of mice irradiated with lethal doses of (60)Co γ radiation (CD2F1, 9.2 Gy) and X-ray exposures (Balb/c, 9 Gy) that are known to cause acute radiation syndrome in hematopoietic tissues. Survival benefit was mMPC-dose dependent and significant even when mMPC administration was delayed up to 7 days after irradiation. We further show that mMPC administration mitigates death from acute radiation syndrome at radiation doses of up to 15 Gy ((60)Co γ radiation, CD2F1), which are radiation exposure levels that cause mice to succumb to multi-organ failure, and determined that the dose-reduction factor of 5 million mMPC administered 24 h after irradiation of CD2F1 mice is 1.73. Even at high doses of up to 14...Continue Reading

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Citations

Oct 6, 2012·Journal of Radiation Research·Vijay K SinghOluseyi O Fatanmi
Jul 3, 2013·Journal of Radiation Research·Vijay K SinghThomas M Seed
Jul 6, 2014·Radiation Protection Dosimetry·Vijay K SinghThomas M Seed
Oct 16, 2014·Expert Opinion on Therapeutic Patents·Vijay K SinghThomas M Seed
Nov 17, 2015·Expert Review of Molecular Diagnostics·Vijay K SinghHarvey B Pollard
Nov 25, 2014·Expert Opinion on Biological Therapy·Vijay K SinghVictoria L Newman
Dec 29, 2012·International Journal of Radiation Biology·Vijay K SinghThomas M Seed
Feb 6, 2015·Frontiers in Oncology·Eliot M RosenVijay K Singh
May 7, 2016·International Journal of Molecular Sciences·Vijay K Singh, Martin Hauer-Jensen
Jun 13, 2017·Radiation Research·Andrea L DiCarloJean-Rene Jourdain
Jun 14, 2017·Radiation Research·Carmen RiosAndrea L DiCarlo
Jan 24, 2018·Scientific Reports·Mitsuru ChibaAndrzej Wojcik
Apr 23, 2019·Expert Opinion on Drug Discovery·Vijay K SinghAyodele O Olabisi
Aug 26, 2020·Stem Cell Reviews and Reports·Selçuk ÖztürkYaşar Murat Elçin
Feb 11, 2020·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Mayuri BandekarSantosh K Sandur
Oct 17, 2020·Drug Discovery Today·Vijay K Singh, Thomas M Seed
Nov 5, 2020·Biomedicines·Elena ObradorAlegría Montoro
Mar 20, 2021·Scientific Reports·Yaoxiang LiVijay K Singh
Jun 29, 2021·Free Radical Research·Rahul CheckerSantosh K Sandur

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Methods Mentioned

BETA
sandwich immunoassay
X-Ray

Software Mentioned

SPSS

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