Myeloma kappa gene transcription is blocked upon fusion with fibroblasts

Experimental Cell Research
S JunkerD Picard

Abstract

Numerous studies on somatic cell hybrids have shown that expression of tissue-specific functions can be suppressed as a consequence of fusion with cells that do not express the given functions. We have further investigated this phenomenon, using as a model system the regulation of expression of kappa light chain genes in intraspecific hybrids between mouse myeloma cells and mouse fibroblasts. Hybrids containing only one genome equivalent from each parent cell (1s:1s) were isolated by fluorescence-activated cell sorting from within 10 h after fusion, and they were grown for no more than 16 days thereafter in order to ensure maximum integrity of the genomic constitution. Here we report that in hybrid cells, kappa gene transcription was specifically turned off as demonstrated by nuclear run-on assays performed on 16-day-old proliferating hybrids. Furthermore, a mechanism affecting mRNA stability may also contribute, at least initially, to the rapid depletion of cytoplasmic kappa transcripts, observed during the first few hours after fusion. Suppression was dominant and could not be overridden by increasing the relative myeloma ploidy at either the heterokaryon or the synkaryon stage. Nor could suppression be relieved by treating h...Continue Reading

References

Nov 1, 1978·Somatic Cell Genetics·D Schall, M Rechsteiner
Aug 1, 1986·Experimental Cell Research·S A Edwards, E D Adamson
Feb 1, 1987·Molecular and Cellular Biology·A GreenbergR Laskov
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Mar 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·S E Malawista, M C Weiss
May 1, 1982·Experimental Cell Research·S Junker
Dec 1, 1984·The Journal of Experimental Medicine·T IshiharaT Watanabe
Oct 15, 1981·Biochemical and Biophysical Research Communications·S Junker, S Pedersen

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