PMID: 2108214Apr 1, 1990Paper

Myelomas and lymphomas expressing the Ig gamma 2a H chain gene have similar transcription termination regions

The Journal of Immunology : Official Journal of the American Association of Immunologists
J A Flaspohler, C Milcarek

Abstract

During B cell differentiation, the membrane and secretion specific forms of the Ig gamma-H chains of mouse are differentially expressed as a function of the developmental stage of the cell. Representatives of less differentiated and memory B cells (lymphomas) that have undergone the class switch to gamma 2a or gamma 2b H chains produce nearly equal amounts of membrane specific (gamma m) vs secretory specific (gamma s) mRNA. Fully differentiated gamma 2a or gamma 2b plasma cells and their tumors, myelomas, switch to higher levels of gamma s mRNA production relative to gamma m. Selective use of either the gamma s poly(A) site or the downstream gamma m poly(A) site accompanied by specific splicing events could modulate production of these two forms of mature gamma H chain mRNA. Alternatively, transcription termination could be modulated. Through a combination of hybrid protection and in vitro nascent RNA analyses of transcripts from gamma H chain-producing cells arrested at various stages of development, we have mapped transcription termination in both lymphomas (gamma s approximately gamma m mRNA) and in myelomas (gamma s much greater than gamma m) mRNA. Regardless of the developmental stage of the cell, transcription proceeds at...Continue Reading

Related Concepts

Related Feeds

B cell Differentiation

Depending on the signal received through the B cell receptor and other receptors, B cells differentiate into follicular or marginal zone B cells. Here is the latest research pertaining to this differentiation process.