Myeloperoxidase evokes substantial vasomotor responses in isolated skeletal muscle arterioles of the rat

Acta Physiologica
Viktória CsatóZoltán Papp

Abstract

Myeloperoxidase (MPO) catalyses the formation of a wide variety of oxidants, including hypochlorous acid (HOCl), and contributes to cardiovascular disease progression. We hypothesized that during its action MPO evokes substantial vasomotor responses. Following exposure to MPO (1.92 mU mL(-1)) in the presence of increasing concentrations of hydrogen peroxide (H2O2), changes in arteriolar diameter of isolated gracilis skeletal muscle arterioles (SMAs) and coronary arterioles (CAs) and in the isometric force in basilar arteries (BAs) of the rat were monitored. Myeloperoxidase increased vascular tone to different degrees in CAs, SMAs and BAs. The mechanism of increased vasoconstriction was studied in detail in SMAs. MPO-evoked vasoconstrictions were prevented by the MPO inhibitor 4-aminobenzhydrazide (50 μM), by endothelium removal in the SMAs. Surprisingly, the HOCl scavenger L-methionine (100 μM), the thromboxane A2 (TXA2) antagonist SQ-29548 (1 μM) or the non-specific cyclooxygenase (COX) antagonist indomethacin (1 μM) converted the MPO-evoked vasoconstrictions to pronounced vasodilations in SMAs, not seen in the presence of H2O2. In contrast to noradrenaline-induced vasoconstrictions, the MPO-evoked vasoconstrictions were not a...Continue Reading

References

Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J M AlbrichJ K Hurst
Jun 1, 1995·The Biochemical Journal·A J KettleC C Winterbourn
Jul 1, 1994·The Journal of Clinical Investigation·A DaughertyJ W Heinecke
Mar 15, 1996·The Journal of Clinical Investigation·L J HazellR Stocker
Aug 1, 1996·Archives of Biochemistry and Biophysics·W A Prütz
Jan 15, 1997·The Biochemical Journal·A J KettleC C Winterbourn
Jun 8, 1999·The Journal of Clinical Investigation·E A PodrezS L Hazen
Apr 8, 2000·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·N N TuranI Kanzik
Aug 18, 2000·Free Radical Biology & Medicine·E A PodrezS L Hazen
Sep 8, 2000·Stroke; a Journal of Cerebral Circulation·Y Iida, Z S Katusic
Nov 25, 2000·The Journal of Biological Chemistry·H M Abu-Soud, S L Hazen
Dec 20, 2000·The Journal of Clinical Investigation·T MatobaA Takeshita
May 1, 2001·IUBMB Life·J M PullarC C Winterbourn
May 26, 2001·The Journal of Biological Chemistry·C ZhangC R White
Sep 15, 2001·American Journal of Physiology. Heart and Circulatory Physiology·C ZhangC R White
Nov 22, 2001·JAMA : the Journal of the American Medical Association·R ZhangS L Hazen
Jun 14, 2002·European Journal of Pharmacology·Robert PórszászJános Szolcsányi
Jun 29, 2002·Science·Jason P EiserichBruce A Freeman
Feb 8, 2003·Circulation Research·Hiroto MiuraDavid D Gutterman
Apr 10, 2003·British Journal of Pharmacology·Yu-Jing GaoRobert M K W Lee
May 24, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Tetsuya MatobaAkira Takeshita
Sep 4, 2003·Circulation·Stephan BaldusUNKNOWN CAPTURE Investigators
Oct 24, 2003·The New England Journal of Medicine·Marie-Luise BrennanStanley L Hazen
Nov 14, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Naris Thengchaisri, Lih Kuo
Nov 14, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Chunxiang ZhangLisa K Jennings
Aug 21, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Akos Koller, Zsolt Bagi
Aug 28, 2004·Arteriosclerosis, Thrombosis, and Vascular Biology·Roland StockerJohn F Keaney
Jan 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tim O HircheAzzaq Belaaouaj
Feb 4, 2005·Journal of Leukocyte Biology·Seymour J Klebanoff
Mar 26, 2005·Arteriosclerosis, Thrombosis, and Vascular Biology·Stephen J Nicholls, Stanley L Hazen
Sep 27, 2005·Archives of Biochemistry and Biophysics·Markus HanssonWilliam M Nauseef
Oct 19, 2005·British Journal of Pharmacology·Yu-Jing Gao, Robert M K W Lee

❮ Previous
Next ❯

Citations

Jan 20, 2017·Acta Physiologica·P B Persson, A B Persson

❮ Previous
Next ❯

Methods Mentioned

BETA
SMAs
SMA

Software Mentioned

Excel
Office
ImageJ

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.