Myeloperoxidase influences the complement regulatory activity of complement factor H.

Rheumatology
Su-Fang ChenMing-Hui Zhao

Abstract

The interaction between neutrophils and activation of alternative complement pathway plays a critical role in the pathogenesis of ANCA-associated vasculitis (AAV). MPO, which can be released from ANCA-stimulated neutrophils, was recently demonstrated to be capable of activating the alternative complement pathway. Here we aimed to investigate the interaction between MPO and factor H (FH), a key regulator of the alternative pathway, and its effect on the functional activities of FH. Detection of FH and MPO on neutrophil extracellular traps (NETs) induced by serum from AAV patients and in kidney biopsies of AAV patients was performed by immunostaining. In vitro binding between MPO and FH was examined by ELISA and surface plasmon resonance. The influence of MPO on the complement regulatory activity of FH was further assessed. FH deposited and co-localized with MPO in NETs. In kidney biopsies from AAV patients, MPO was closely adjacent to FH in glomerular capillaries. We demonstrated that MPO binds to FH with an apparent nanomolar affinity and identified short consensus repeats 1-4 of FH as the major binding sites. In terms of functional analysis, MPO inhibited the interaction between FH and C3b and the decay-accelerating activity o...Continue Reading

References

Sep 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·J M WeilerD T Fearon
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R D HorstmannV A Fischetti
May 1, 1987·The Journal of Clinical Investigation·R J JohnsonS J Klebanoff
Oct 24, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Viviana P FerreiraMichael K Pangburn
Oct 23, 2008·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Min ChenMing-Hui Zhao
Dec 4, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Livija DebanSeppo Meri
Dec 17, 2008·Journal of the American Society of Nephrology : JASN·Adrian SchreiberRalph Kettritz
Dec 6, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Karita HaapasaloT Sakari Jokiranta
Sep 4, 2012·Journal of the American Society of Nephrology : JASN·Lise Halbwachs, Philippe Lesavre
Mar 28, 2013·The Journal of Biological Chemistry·Uwe JerkeRalph Kettritz
May 25, 2013·Biochemistry·Elisavet MakouPaul N Barlow
Jun 19, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mario HebeckerMihály Józsi
Oct 12, 2013·Clinical Journal of the American Society of Nephrology : CJASN·Shen-Ju GouMin Chen
Oct 25, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lin ZouWei Chao
Nov 2, 2013·Journal of the American Society of Nephrology : JASN·Hong XiaoJ Charles Jennette
Dec 21, 2013·Journal of Innate Immunity·Joseph O'FlynnCees van Kooten
May 13, 2015·Clinical and Experimental Immunology·H WangM Chen
Nov 19, 2015·Kidney International·William G Couser, Richard J Johnson
Dec 13, 2016·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Su-Fang ChenMing-Hui Zhao

❮ Previous
Next ❯

Citations

May 7, 2020·Frontiers in Immunology·Lani ShochetA Richard Kitching
Nov 18, 2019·Autoimmunity Reviews·Benoit BrillandJean-François Augusto
Oct 22, 2021·Frontiers in Immunology·Éva KárpátiMihály Józsi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alternative Complement Pathway

The Alternative Complement Pathway is part of the innate immune system, and activation generates membrane attack complexes that kill pathogenic cells. Discover the latest research on the Alternative Complement Pathway.