Myeloperoxidase mRNA analysis in acute lymphoblastic leukemia

Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B
D CrisanB O'Malley

Abstract

Expression of the myeloperoxidase (MPO) gene at the mRNA level is a better lineage marker than enzymatic activity in early myeloid precursors and their leukemic counterparts. Its diagnostic use depends on the specificity of expression for myeloblasts and its absence in blasts of lymphoid lineage. The present study investigates MPO mRNA expression in adult acute lymphoblastic leukemia (ALL), using reverse transcription-polymerase chain reaction. Of a total of 13 cases, six were found to have blasts positive for MPO mRNA; in all of these cases, the blasts were cytochemically negative for MPO. This unexpected finding of MPO mRNA positivity in six of 13 cases was further investigated at the molecular level. Bcr gene rearrangement analysis was positive in all six cases for the bcr breakpoint diagnostic of chronic myelogenous leukemia (CML). Only three of these six cases were cytogenetically positive for a Philadelphia (Ph) chromosome. Based on molecular analysis, these cases are considered as CML presenting in blast crisis of lymphoid lineage, as opposed to de novo ALL. The remaining seven cases were Ph negative at the cytogenetic and molecular levels; the leukemic blasts were MPO mRNA negative, confirming the lack of MPO gene expre...Continue Reading

References

Oct 1, 1990·Journal of Clinical Pathology·J StorrJ T Reilly
Mar 21, 1991·The New England Journal of Medicine·S R WiersmaK I Weinberg
Feb 1, 1991·Annals of Hematology·D CatovskyR Morilla
Aug 1, 1990·British Journal of Haematology·K Dijkstra, H C Kluin-Nelemans
Nov 1, 1990·American Journal of Clinical Pathology·C W RossC A Hanson
Mar 1, 1989·Clinical Immunology and Immunopathology·S R ZakiG E Austin
Oct 11, 1989·Nucleic Acids Research·K JohnsonG Rovera
May 18, 1989·Nature·S Kwok, R Higuchi
Jan 1, 1988·British Journal of Haematology·G HeilH Heimpel
Feb 1, 1988·European Journal of Haematology·W M NauseefK Arnljots
Aug 1, 1988·Journal of Cellular Physiology·A ToblerH P Koeffler
May 15, 1987·Archives of Biochemistry and Biophysics·M YamadaS Tsunasawa
Jul 1, 1985·British Journal of Haematology·W C PughJ D Rowley
Jun 1, 1993·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·D CrisanJ R Krause
Mar 1, 1993·European Journal of Haematology·P LepelleyA Cosson

❮ Previous
Next ❯

Citations

Mar 28, 2013·Archives of Pathology & Laboratory Medicine·Jane ZhouL Jeffrey Medeiros
Jul 17, 2009·American Journal of Clinical Pathology·Michael E RyttingMaher Albitar
Jul 13, 2001·American Journal of Clinical Pathology·D A ArberM L Slovak
Jul 2, 1999·Blood Cells, Molecules & Diseases·R Stasi, S Amadori
Jun 1, 1997·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·J SerranoA Torres
Jul 27, 2021·Clinics in Laboratory Medicine·Nidhi Aggarwal, Olga K Weinberg

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.