Jan 31, 1976

Myo-inositol oxygenase from oat seedlings

Molecular and Cellular Biochemistry
E KollerO Hoffmann-Ostenhof

Abstract

Enzyme preparations from oat seedlings showing the activity of myo-inositol oxygenase (E.C.1.13.99.1) have been described previously. In contrast to myo-inositol oxygenase preparations from other sources, e.g. rat kidney or yeast, the oat enzyme seemed to exhibit a somewhat less stringent activity, acting on other inositols and inositol methyl ethers as well as on myo-inositol. By purification of the enzyme present in the extract from oat seedlings with the help of an affinity gel specific for enzymes acting on myo-inositol a homogeneous enzyme preparation was obtained, which shows the same strict specificity as the myo-inositol oxygenase from other sources. It has a molecular weight of 62,000 and tends to aggregate to oligomers (up to tetramers) under physiological pH-values; in more alkaline media dissociation to monomers is observed. The action on the other inositols and inositol methyl ethers is apparently due to one or more other enzymes, which are also adsorbed on the affinity gel, but can be separated from the myo-inositol oxygenase by elution with increasing concentrations of myo-inositol.

  • References1
  • Citations8

References

Mentioned in this Paper

Triticale
Methyl Ethers
Enzymes, antithrombotic
Structure-Activity Relationship
Enzyme agent
Enzymes for Treatment of Wounds and Ulcers
Enzyme preparations, digestives
Purification Aspects
Miox
MIOX gene

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