myo-Inositol uptake is essential for bulk inositol phospholipid but not glycosylphosphatidylinositol synthesis in Trypanosoma brucei.

The Journal of Biological Chemistry
Amaia Gonzalez-SalgadoPeter Bütikofer

Abstract

myo-Inositol is an essential precursor for the production of inositol phosphates and inositol phospholipids in all eukaryotes. Intracellular myo-inositol is generated by de novo synthesis from glucose 6-phosphate or is provided from the environment via myo-inositol symporters. We show that in Trypanosoma brucei, the causative pathogen of human African sleeping sickness and nagana in domestic animals, myo-inositol is taken up via a specific proton-coupled electrogenic symport and that this transport is essential for parasite survival in culture. Down-regulation of the myo-inositol transporter using RNA interference inhibited uptake of myo-inositol and blocked the synthesis of the myo-inositol-containing phospholipids, phosphatidylinositol and inositol phosphorylceramide; in contrast, it had no effect on glycosylphosphatidylinositol production. This together with the unexpected localization of the myo-inositol transporter in both the plasma membrane and the Golgi demonstrate that metabolism of endogenous and exogenous myo-inositol in T. brucei is strictly segregated.

References

Oct 1, 1995·Molecular and Cellular Biology·M E DrewS M Landfear
Jan 1, 1993·Trends in Biochemical Sciences·M D Marger, M H Saier
Apr 28, 2000·European Journal of Biochemistry·M Einicker-LamasM M Oliveira
Jul 4, 2002·The Journal of Biological Chemistry·Natacha Bochud-Allemann, André Schneider
Jul 23, 2002·The Journal of Biological Chemistry·Michael J CoadyJean-Yves Lapointe
Feb 13, 2003·The Journal of Biological Chemistry·Gerard T BerryJohn J Greer
Apr 23, 2003·Molecular and Biochemical Parasitology·Seth RedmondMark C Field
Oct 11, 2003·FEBS Letters·Arun Lahiri MajumderManoj Majee
Apr 28, 2004·Journal of Molecular Biology·Lukas KällErik L L Sonnhammer
May 13, 2004·The Journal of Cell Biology·Cynthia Y HeGraham Warren
Feb 11, 2005·Molecular Nutrition & Food Research·Erwin Sigel, Frédéric Minier
Sep 22, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jenny F L ChauStephen S M Chung
Oct 26, 2005·Biochemical Society Transactions·K L Martin, T K Smith
Nov 5, 2005·Molecular and Biochemical Parasitology·Michael OberholzerThomas Seebeck
Feb 16, 2006·The Biochemical Journal·Kirstee L Martin, Terry K Smith
Jul 25, 2006·Biochimica Et Biophysica Acta·Martine Pittet, Andreas Conzelmann
Oct 13, 2006·Nature·Gilbert Di Paolo, Pietro De Camilli
Nov 24, 2006·Sub-cellular Biochemistry·Frank A Loewus
Jan 16, 2007·Journal of Internal Medicine·E M WrightD F Loo
Mar 10, 2007·Microbiology and Molecular Biology Reviews : MMBR·Yosuke Koga, Hiroyuki Morii
Jun 26, 2007·Experimental Parasitology·Marcelo Einicker-LamasCelso Caruso-Neves
Sep 12, 2007·Bioinformatics·M A LarkinD G Higgins
Nov 24, 2007·BMC Bioinformatics·Daniel H HusonRegula Rupp
Jan 25, 2008·Nature Reviews. Molecular Cell Biology·Robert H Michell
May 3, 2008·Bipolar Disorders·Yuly BersudskyRobert H Belmaker
May 30, 2008·Frontiers in Bioscience : a Journal and Virtual Library·Xueli Guan, Markus R Wenk
Jun 10, 2008·Annual Review of Microbiology·Christopher J LawDa-Neng Wang
Aug 14, 2008·Molecular Microbiology·Shaheen S SutterwalaJames D Bangs
Nov 22, 2008·Nucleic Acids Research·Milton H SaierCharles Elkan

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Citations

Mar 31, 2015·FEBS Letters·Sabine Schneider
Jul 13, 2013·Biochimica Et Biophysica Acta·Kentaro Hanada
Mar 8, 2016·Biochimica Et Biophysica Acta·Neha ChauhanPeter Bütikofer
Sep 27, 2012·Biochimica Et Biophysica Acta·Luce Farine, Peter Bütikofer
Jul 6, 2013·Progress in Lipid Research·Srinivasan RamakrishnanPeter Bütikofer
Jan 4, 2017·PloS One·Christoph MathieuDoris Rentsch
Oct 25, 2017·The FEBS Journal·Remo S SchmidtPascal Mäser
Apr 7, 2018·Molecular Microbiology·Mariana Galvao FerrariniMarie-France Sagot
Aug 3, 2017·Clinical and Translational Medicine·Michael BiagiottiRachel Zufferey
Apr 15, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Michael E SteinmannErwin Sigel
Nov 23, 2019·Proceedings of the National Academy of Sciences of the United States of America·Yann DesfougèresAdolfo Saiardi
Feb 12, 2017·Journal of Bioenergetics and Biomembranes·Thais Russo-AbrahãoJosé Roberto Meyer-Fernandes
Dec 22, 2017·Emerging Topics in Life Sciences·Snezhana Akpunarlieva, Richard Burchmore
Jan 8, 2021·ACS Chemical Biology·Brian S MantillaRoberto Docampo
Sep 6, 2012·Analytical Chemistry·Darren J CreekMichael P Barrett
Oct 16, 2021·PloS One·Bryan C JensenMarilyn Parsons

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