Myocardial connexin-43 and PKC signalling are involved in adaptation of the heart to irradiation-induced injury: Implication of miR-1 and miR-21

General Physiology and Biophysics
Csilla ViczenczováN Tribulova

Abstract

Intercellular connexin-43 (Cx43) channels are essential for electrical coupling and direct cardiac cell to cell communication to ensure heart function. Expression of Cx43 is altered due to stressful conditions and also affected by the alterations in extracellular matrix. We aimed to explore the effect of chest irradiation on myocardial expression of Cx43 and miR-1 which regulates GJA1 gene transcription for Cx43. Implication of miR-21 that regulates expression of extracellular matrix proteins and PKC signalling that may affect Cx43-mediated coupling was examined as well. Western blot and real-time PCR analyses revealed that six weeks after the exposure of healthy Wistar rats chest to single irradiation of 25 Gy significant myocardial alterations were observed: 1)/ increase of total Cx43 protein expression and its functional phosphorylated forms; 2) suppressed levels of miR-1; 3) enhanced expression of PKCε which phosphorylates Cx43; 4) increase of miR-21 levels; 5) increase of PKCδ expression. These results suggest that irradiation causes post-transcriptional regulation of myocardial Cx43 expression by miR-1 possibly through miR-21 and PKC signalling. We conclude that single dose of irradiation has the potential to enhance myoc...Continue Reading

Citations

Jun 5, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tyler W LeBaronJan Slezak
Jul 28, 2017·Canadian Journal of Physiology and Pharmacology·Jan SlezakNarcis Tribulova
Jul 6, 2017·Canadian Journal of Physiology and Pharmacology·Branislav KuraJan Slezak
May 2, 2017·Canadian Journal of Physiology and Pharmacology·Csilla ViczenczovaJan Slezak
Jan 25, 2020·International Journal of Molecular Sciences·Branislav KuraMonika Bartekova
Jun 15, 2020·Journal of Cellular and Molecular Medicine·Bin WangXin Jiang
Apr 13, 2018·International Journal of Molecular Sciences·Csilla ViczenczovaBarbara Szeiffova Bacova
Mar 30, 2019·Journal of Cancer Research and Clinical Oncology·Peter G HawkinsShruti Jolly
Mar 21, 2020·Cancers·Laura PellegriniAlessandra Magenta
Mar 5, 2021·Journal of the American Heart Association·Myung-Jin ChaJi Hyun Chang

❮ Previous
Next ❯

Related Concepts