Myocardial gene expression associated with genetic cardiac hypertrophy in the absence of hypertension

Hypertension Research : Official Journal of the Japanese Society of Hypertension
Jeremy P DwyerR Di Nicolantonio

Abstract

The hypertrophic heart rat (HHR) was derived from the spontaneously hypertensive rat of the Okamoto strain and develops cardiac hypertrophy in the absence of hypertension. The genetic basis of this hypertrophy is unknown. Therefore, we compared gene expression profiles in the left ventricular myocardium of young (8-10 weeks of age) and old (38-50 weeks) HHR with rats from an age-matched control strain, the normal heart rat (NHR). cDNA microarrays (National Institute of Aging [NIA], 15,247 clones) were used to evaluate gene expression in cardiac-derived Cy3 and Cy5 labeled cDNA. M values (log2[Cy5/Cy3]) were obtained and significant differential expression was identified using an empirical Bayesian approach with specific results verified using real-time PCR. Compared with NHR, HHR cardiac weight index (heart weight/ body weight) was significantly elevated at both ages (young: 5.5 +/- 0.5 vs. 3.9 +/- 0.2; old: 4.2 +/- 0.3 vs. 3.4 +/- 0.2 mg/g; p < 0.05) with no difference in body weight or in tail-cuff blood pressure detected between the strains at either age. Differential expression was observed in 65 and 390 clones in young and old HHR, respectively, with more genes exhibiting down-regulation than up-regulation in both instance...Continue Reading

References

Jul 10, 1991·JAMA : the Journal of the American Medical Association·M S LauerD Levy
Dec 14, 1987·The American Journal of Cardiology·W B KannelD Levy
Jan 1, 1996·European Heart Journal·J TingleffM Kirchoff
Aug 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·M RotheD V Goeddel
Oct 1, 1996·Molecular and Cellular Biology·D E AyerR N Eisenman
May 1, 1997·The American Journal of Physiology·J P BénitahW J Lederer
Feb 28, 1998·Journal of Molecular and Cellular Cardiology·M A HeftiM C Schaub
Feb 1, 2000·Biologicals : Journal of the International Association of Biological Standardization·H Shams, I Heron
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·C J FriddleJ Bristow
Jun 9, 2000·Annual Review of Physiology·W R MacLellan, M D Schneider
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·T S TanakaM S Ko
Oct 26, 2000·Trends in Biochemical Sciences·S Raha, B H Robinson
Feb 24, 2001·Molecular Pathology : MP·A M SnijdersP J van Diest
Jun 8, 2001·Proceedings of the National Academy of Sciences of the United States of America·N H PurcellA Lin
Oct 18, 2001·Journal of Molecular and Cellular Cardiology·A A DempseyC C Liew
Feb 8, 2002·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·C R SchererK Steinmeyer
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Apr 12, 2002·Physiological Genomics·Stephen B HarrapLeanne M D Delbridge
Oct 22, 2002·American Journal of Physiology. Heart and Circulatory Physiology·James D Stockand, J Gary Meszaros
Nov 15, 2002·Journal of Molecular and Cellular Cardiology·Philip EatonMichael J Shattock
Dec 3, 2002·Revista española de cardiología·José Marín-García, Michael J Goldenthal
Jan 10, 2003·Nucleic Acids Research·Maximilian DiehnAsh A Alizadeh
Apr 25, 2003·Methods in Molecular Biology·Gordon K SmythTerry Speed
Nov 5, 2003·Methods : a Companion to Methods in Enzymology·Gordon K Smyth, Terry Speed
Dec 18, 2003·Clinical and Experimental Pharmacology & Physiology·Ping Ye, Malcolm J West
Jun 25, 2004·Journal of Pharmacological Sciences·Seongchun KwonHee Yul Ahn
Mar 8, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Sudhiranjan GuptaSubha Sen
May 3, 2005·The Lancet Oncology·Jing Yuan Fang, Bruce C Richardson

❮ Previous
Next ❯

Citations

Jul 17, 2009·Journal of Proteome Research·Jennifer E GrantKevin L Schey
Jun 2, 2016·Biological Reviews of the Cambridge Philosophical Society·Shahzad SadiqPaul A Lewandowski
Dec 2, 2010·Nature Reviews. Drug Discovery·Friedrich GrimmingerHossein A Ghofrani
May 12, 2012·Molecular Medicine·Valentina SalaTiziana Crepaldi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiomegaly

Cardiomegaly, known as an enlarged heart, is a multifactorial disease with different pathophysiological mechanisms. Hypertension, pregnancy, exercise-induced and idiopathic causes are some mechanisms of cardiomegaly. Discover the latest research of cardiomegaly here.

Cardiac Remodeling

Cardiac remodeling in response to a myocardial infarction is characterized by progressive ventricular dilatation, cardiac hypertrophy, fibrosis, and deterioration of cardiac performance. Discover the latest research on Cardiac Remodeling here.