Myocardial gene expression of angiogenic factors in human chronic ischemic myocardium: influence of acute ischemia/cardioplegia and reperfusion

Microcirculation : the Official Journal of the Microcirculatory Society, Inc
Yongzhong WangJens Kastrup

Abstract

Angiogenic therapies in animals have demonstrated the development of new blood vessels within ischemic myocardium. However, results from clinical protein and gene angiogenic trials have been less impressive. The present study aimed to investigate the expression of angiogenic genes in human chronic ischemic myocardium and the influence of acute ischemia/cardioplegia and reperfusion on their expression. Myocardial biopsies were taken from chronic ischemic and nonischemic myocardium in 15 patients with stable angina pectoris during coronary bypass surgery. Tissue samples were evaluated by oligonucleotide microarray and quantitative real-time PCR for the expression of angiogenic factors. There was identical baseline expression of VEGF-A and VEGF-C mRNA in chronic ischemic myocardium compared with nonischemic myocardium. Reperfusion increased the gene expression of VEGF-A and VEGF-C mRNA both in nonischemic and ischemic myocardium. VEGF-A protein was detected mainly in the extracellular matrix around the cardiomyocytes in ischemic myocardium. These data suggest that the nonconclusive VEGF gene therapy trials chronic coronary artery disease was not due to a preexisting upregulation of VEGF in chronic ischemic myocardium. There might ...Continue Reading

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Citations

Jan 16, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Jian-Xiong ChenJudy L Aschner
Jul 31, 2010·Expert Review of Cardiovascular Therapy·Jens Kastrup
Jan 21, 2016·Expert Opinion on Drug Delivery·Taiyoun Rhim, Minhyung Lee
Apr 14, 2011·Scandinavian Cardiovascular Journal : SCJ·Tina FriisJens Kastrup
Jul 11, 2008·Scandinavian Cardiovascular Journal : SCJ·Yongzhong WangJens Kastrup
Jan 19, 2011·Advanced Drug Delivery Reviews·Hyun Ah KimMinhyung Lee
Nov 21, 2008·Journal of Internal Medicine·S VööJ Waltenberger
Oct 5, 2010·Journal of the American College of Cardiology·Nirat BeoharDouglas W Losordo
Nov 18, 2006·Journal of Molecular and Cellular Cardiology·Jason C KovacicRobert M Graham
Apr 15, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Qin-Hui TuoJian-Xiong Chen
Sep 5, 2018·Chinese Journal of Integrative Medicine·Lan MaWei Mao

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