Myocardial glucose utilization. Failure of adenosine to alter it and inhibition by the adenosine analogue N6-(L-2-phenylisopropyl)adenosine

Circulation Research
W E DaleM J Rovetto

Abstract

The effects of adenosine and the nonmetabolizable adenosine analogue N6-(L-2-phenylisopropyl)adenosine (PIA) on glucose transport or metabolism were determined in purified myocardial sarcolemmal vesicles, isolated cardiocytes, and perfused hearts. Adenosine (100 microM) did not affect hexose transport in myocytes. Also, adenosine deaminase, added to metabolize adenosine to inosine, did not alter transport of hexose into myocytes regardless of whether or not insulin was present. In contrast, PIA effectively inhibited 3-O-methyl-D-glucose uptake in myocytes even during insulin stimulation. PIA inhibited D-glucose-specific transport in both rat and bovine cardiac sarcolemmal vesicles (Ki = 26 microM at [D-glucose] = 5 mM). However, insulin did not affect glucose transport in sarcolemmal vesicles, which implies that receptor-coupled processes probably are not intact in this preparation. Thus, inhibition of PIA may not be receptor mediated. Also, PIA inhibited binding of cytochalasin B to bovine cardiac sarcolemmal vesicles, which supports the idea that PIA inhibits glucose flux by binding to the glucose transporter. To determine if adenosine altered glucose metabolism rather than transport, we measured the rate of 3H2O production f...Continue Reading

References

May 1, 1979·The American Journal of Physiology·M J Seider, H D Kim
Mar 1, 1978·Journal of Molecular and Cellular Cardiology·G J JesmokH F Hardman
Jul 4, 1978·Biochimica Et Biophysica Acta·I BihlerI G Sloan
Jan 1, 1975·Methods in Enzymology·J R Neely, M J Rovetto
Sep 1, 1987·Naunyn-Schmiedeberg's Archives of Pharmacology·D MartensU Schwabe
Jun 1, 1987·Pflügers Archiv : European journal of physiology·T P GeisbuhlerM J Rovetto
Oct 25, 1972·Biochimica Et Biophysica Acta·D L Ford, A Omachi
Jan 1, 1967·Biochemistry·J Katz, A Dunn
Aug 24, 1983·Biochimica Et Biophysica Acta·K D Philipson, A Y Nishimoto
Feb 15, 1983·Archives of Biochemistry and Biophysics·C M HohlG P Brierley
Jan 1, 1972·FEBS Letters·V C. De Sanchez, E Piña

Citations

Jun 6, 1998·The Journal of Biological Chemistry·S K SrivastavaA Bhatnagar
Jan 13, 2000·Journal of Basic and Clinical Physiology and Pharmacology·D El-AniA Shainberg
Oct 11, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Irina ShuralyovaImogen R Coe
Oct 1, 1993·The American Journal of Physiology·E MurphyC Steenbergen
May 1, 1997·The American Journal of Physiology·S A GabelR E London
Jan 25, 2000·American Journal of Physiology. Endocrinology and Metabolism·E O McFallsH B Ward

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.

Glut1 Deficiency

Glut1 deficiency, an autosomal dominant, genetic metabolic disorder associated with a deficiency of GLUT1, the protein that transports glucose across the blood brain barrier, is characterized by mental and motor developmental delays and infantile seizures. Follow the latest research on Glut1 deficiency with this feed.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Separation Anxiety

Separation anxiety is a type of anxiety disorder that involves excessive distress and anxiety with separation. This may include separation from places or people to which they have a strong emotional connection with. It often affects children more than adults. Here is the latest research on separation anxiety.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.