Myocardial perfusion imaging is feasible for infarct size quantification in mice using a clinical single-photon emission computed tomography system equipped with pinhole collimators.

Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging
Tim WollenweberMarcus Hacker

Abstract

The aim of this study is to evaluate a non-invasive method for measuring myocardial perfusion defect size in mice using a clinical single-photon emission computed tomography system equipped with pinhole collimators (pinhole SPECT). Thirty days after ligation of the left anterior descending coronary artery, 13 mice (C57BL/6J) were imaged following intravenous injection of 370 MBq [99mTc]sestamibi. Eight control mice without myocardial infarction were likewise investigated. Image quality optimization had been achieved by repeated scanning of a multiple point phantom, with varying zoom factors, number of projection angles, and pinhole diameter. Volumetric sampling was used to generate polar maps, in which intensity was normalized to that of a standard septal region of interest (ROI), which was set at 100%. Receiver operating characteristic analyses were performed to define an optimal threshold as compared to histologically measured defect sizes, which were considered as gold standard. A spatial resolution of 1.9 mm was achieved using a pinhole diameter of 0.5 mm, a zoom factor of 2, and 6 degrees projection angles. Histological results were best reproduced by a 60% threshold relative to the septal reference ROI. By applying this t...Continue Reading

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Citations

Sep 6, 2014·Journal of Nuclear Cardiology : Official Publication of the American Society of Nuclear Cardiology·Andrei TodicaSebastian Lehner
Feb 15, 2013·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Andrei TodicaMarcus Hacker
Mar 8, 2012·Journal of Nuclear Cardiology : Official Publication of the American Society of Nuclear Cardiology·Adelaide GrecoAlberto Cuocolo
Apr 17, 2012·Journal of Biomedicine & Biotechnology·Sara GargiuloAlberto Cuocolo
Feb 25, 2015·World Journal of Nuclear Medicine·Jalil Pirayesh IslamianEsmail Gharapapagh
Nov 27, 2014·World Journal of Stem Cells·Bruno C HuberStefan Brunner
Jul 12, 2019·Journal of Nuclear Cardiology : Official Publication of the American Society of Nuclear Cardiology·Antti SarasteAnne Roivainen
Apr 21, 2017·Frontiers in Medicine·Francesco CiconeJohn O Prior
Nov 3, 2012·Experimental Physiology·Adelaide GrecoAlberto Cuocolo
Jul 20, 2017·Journal of Nuclear Cardiology : Official Publication of the American Society of Nuclear Cardiology·Adam Ali GhotbiAndreas Kjaer
May 3, 2019·Journal of Nuclear Cardiology : Official Publication of the American Society of Nuclear Cardiology·Annika HessJames T Thackeray

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