MyoD is indispensable for muscle-specific alternative splicing in mouse mitochondrial ATP synthase gamma-subunit pre-mRNA.

The Journal of Biological Chemistry
M IchidaY Kagawa

Abstract

Muscle-specific alternative RNA splicing is an essential step during myogenesis. In this paper, we report that a muscle-specific transcription factor, MyoD, plays a central role in the induction of muscle-specific alternative splicing during myogenesis. Recently, we reported that muscle and nonmuscle isoforms of the mitochondrial ATP synthase gamma-subunit (F1gamma) were generated by alternative splicing and that acidic stimulation promoted this muscle-specific alternative splicing (Endo, H., Matsuda, C., and Kagawa, Y. (1994) J. Biol. Chem. 269, 12488-12493). In this report, mouse myoblasts are shown to express the muscle-specific isoform of F1gamma after induction with low-serum medium (differentiation medium) or acidic medium, although myotube formation was not detected after acidic induction. RNA blot analysis revealed that the expression levels of both MEF2 and myogenin were increased by low-serum induction, but not by acidic induction. High expression of MyoD mRNA was observed after both types of induction. Overexpression of exogenous MyoD in fibroblasts showed that MyoD was necessary for muscle-specific alternative splicing in both types of induction. Exogenous Id, a negative regulator of MyoD, blocked muscle-specific al...Continue Reading

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Citations

Dec 22, 1999·Biochemical and Biophysical Research Communications·Y KagawaH Endo
May 30, 2001·Advanced Drug Delivery Reviews·Y KagawaH Endo
Jan 20, 2004·Molecular and Cellular Biology·Eiji SakashitaAkila Mayeda
Jan 21, 2004·Developmental Biology·Jeffrey D Amack, Mani S Mahadevan
Dec 18, 2001·The Journal of Biological Chemistry·Morisada HayakawaHitoshi Endo
Oct 22, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Shiro ItoiShugo Watabe
Aug 27, 1999·Advances in Biophysics·Y Kagawa

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