Myofibril assembly is linked with vinculin, alpha-actinin, and cell-substrate contacts in embryonic cardiac myocytes in vitro

Cell Motility and the Cytoskeleton
M TeraiY Shimada

Abstract

The relationship of nascent myofibrils with the accumulation of adhesion plaque proteins and the formation of focal cell contacts was studied in embryonic chick cardiac myocytes in vitro. The cultures were double-stained with various combinations of the specific antiactin drug phalloidin and antibodies against vinculin, alpha-actinin, connectin (titin), myosin heavy chain, fibronectin, and desmin and examined under fluorescence and interference reflection microscopy. In the areas of myofibril assembly, vinculin and alpha-actinin plaques were formed at the ventral sarcolemmae. These areas overlapped with the sites of cell-to-substrate focal contacts and extracellular fibronectin. Because the myofibrils always ran in a straight line between these sites, polarized lines appeared to be generated within the cells in response to their physical (e.g., stress) and/or biochemical environment (e.g., adhesion plaque proteins). The possible presence of other factors cannot be ruled out for the proper alignment of myofibrils. As soon as myofibrils came to span between these adhesion sites, they exhibited typically mature cross-striated characteristics. Thus, the formation of these inferred lines has some relation to, or is in fact necessary...Continue Reading

References

Jul 1, 1978·Journal of Molecular and Cellular Cardiology·L E ThornellM Sjöström
Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·K WangA Tu
Jan 1, 1985·Annals of the New York Academy of Sciences·H HoltzerG Dubyak
Jan 1, 1986·International Review of Cytology·K Maruyama
Jan 1, 1982·Cold Spring Harbor Symposia on Quantitative Biology·B Geiger
Oct 1, 1984·The Journal of Cell Biology·K MaruyamaY Umazume
Jan 1, 1983·Cell and Tissue Research·Y Isobe, Y Shimada
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·B GeigerS J Singer
Aug 31, 1982·Biochemical and Biophysical Research Communications·T Endo, T Masaki
Jan 1, 1982·Cold Spring Harbor Symposia on Quantitative Biology·R O HynesD D Wagner

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Citations

Aug 26, 1998·Cell Motility and the Cytoskeleton·C L Moncman, K Wang
Apr 1, 1993·Developmental Dynamics : an Official Publication of the American Association of Anatomists·M KomiyamaY Shimada
Apr 1, 1991·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A B Fulton, W B Isaacs
Jan 1, 1992·Cell Motility and the Cytoskeleton·L L HilenskiT K Borg
Jan 1, 1993·Cell Motility and the Cytoskeleton·D A SchaferJ A Cooper
Jul 9, 1999·Reviews of Physiology, Biochemistry and Pharmacology·M GautelP Young
Jul 9, 1999·Reviews of Physiology, Biochemistry and Pharmacology·A B Fulton
Oct 19, 1995·Biochimica Et Biophysica Acta·A HattoriR Tatsumi
Jun 1, 1992·The Journal of Cell Biology·M H LuH Holtzer
Sep 1, 1992·The Journal of Cell Biology·B A DanowskiJ W Sanger
Apr 8, 2011·Birth Defects Research. Part A, Clinical and Molecular Teratology·Seungwoo JungTeresa M Gunn
Jan 1, 1992·Current Biology : CB·E Fyrberg
May 5, 2016·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Yotam Blech-HermoniAndrea N Ladd
Oct 1, 1990·Journal of Muscle Research and Cell Motility·M KomiyamaY Shimada

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