Myofibril-bound serine protease and its endogenous inhibitor in mouse: extraction, partial characterization and effect on myofibrils

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
M P SangorrínJorge J Sánchez

Abstract

The protein content of muscle is determined by the relative rates of synthesis and degradation. The balance between this process determines the number of functional contractile units within each muscle cell. Myofibril-bound protease, protease M previously reported in mouse skeletal muscle could be solubilized from the myofibrillar fraction by salt and acid treatment and partially purified by Mono Q and Superose 12 chromatography. Isolated protease M activity in vitro on whole myofibrils resulted in myosin, actin, troponin T, alpha-actinin and tropomyosin degradation. Protease M is serine type and was able to hydrolyze trypsin-type synthetic substrates but not those of chymotrypsin type. In gel filtration chromatography, protease M showed Mr 120.0 kDa. The endogenous inhibitor (MHPI) is a glycoprotein (110.0 kDa) that efficiently blocks the protease M-dependent proteolysis of myofibrillar proteins in a dose-dependent way, as shown by electrophoretic analysis and synthetic substrates assays. Protease M-Inhibitor system would be implicated in myofibrillar proteins turnover.

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Citations

Dec 31, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Makoto OhkuboTadashi Ishihara
Jul 8, 2003·The Journal of Physiology·Eric J StevensonSusan C Kandarian
Apr 1, 2010·Journal of the Science of Food and Agriculture·Penprapha Piyadhammaviboon, Jirawat Yongsawatdigul
Feb 7, 2014·Critical Reviews in Food Science and Nutrition·Nafiseh Soltanizadeh, Mahdi Kadivar
Nov 5, 2015·The Annals of Otology, Rhinology, and Laryngology·Khadijeh Bijangi-VishehsaraeiStacey L Halum
Jun 25, 2005·Journal of Applied Physiology·Carl A MorrisH Lee Sweeney

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