Myofibroblasts and arteriolar sclerosis in human diabetic nephropathy

American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation
E PedagogosG J Becker

Abstract

We examined the biopsy specimens of 62 patients with diabetic nephropathy to establish whether the myofibroblast (MF) has a role in progressive interstitial fibrosis and to ascertain whether a relationship existed between MF activity and severity of arteriolosclerosis. MF were identified by morphology and alpha smooth muscle actin (alpha SMA) immunostaining. Analysis of vascular injury was performed by counting the number of interstitial arterioles after staining endothelial cells with von Willebrand factor (VWF) antibody. Arteriosclerosis was quantified by using a computer-aided image analyzer to measure the arteriolar wall surface and total arteriolar surface area, and the ratio of wall to total surface area was expressed as the index of arteriosclerosis (IA). Fractional area of interstitium (IFA), alpha SMA, and collagen III (Coll III) were quantitated by point counting. Results were related to structural and functional parameters using rank correlation coefficients. There was a strong correlation between IFA and Coll III staining (r = 0.83; P < 0.001). The alpha SMA staining correlated with IFA (r = 0.56; P < 0.001) and Coll III (r = 0.47; P < 0.001), and there were significant correlations between alpha SMA and total urina...Continue Reading

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Citations

Jun 3, 2005·Journal of the American Society of Nephrology : JASN·Josephine M ForbesMark E Cooper
Apr 10, 2020·International Journal of Molecular Sciences·Chenyu Huang, Rei Ogawa
Jun 8, 2006·Nephrology·Duy NguyenSteven J Chadban
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