Myofilament proteins in the synchronous flight muscles of Manduca sexta show both similarities and differences to Drosophila melanogaster

Insect Biochemistry and Molecular Biology
Agnes Ayme-SouthgateDiana Fulmer

Abstract

Insect flight muscles have been classified as either synchronous or asynchronous based on the coupling between excitation and contraction. In the moth Manduca sexta, the flight muscles are synchronous and do not display stretch activation, which is a property of asynchronous muscles. We annotated the M. sexta genes encoding the major myofibrillar proteins and analyzed their isoform pattern and expression. Comparison with the homologous genes in Drosophila melanogaster indicates both difference and similarities. For proteins such as myosin heavy chain, tropomyosin, and troponin I the availability and number of potential variants generated by alternative spicing is mostly conserved between the two insects. The exon usage associated with flight muscles indicates that some exon sets are similarly used in the two insects, whereas others diverge. For actin the number of individual genes is different and there is no evidence for a flight muscle specific isoform. In contrast for troponin C, the number of genes is similar, as well as the isoform composition in flight muscles despite the different calcium regulation. Both troponin I and tropomyosin can include COOH-terminal hydrophobic extensions similar to tropomyosinH and troponinH fou...Continue Reading

References

May 5, 1978·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·J W Pringle
Dec 5, 1988·Journal of Molecular Biology·B BullardE Fyrberg
May 1, 1993·The Journal of Cell Biology·J O VigoreauxM L Pardue
Mar 20, 2002·Nature Cell Biology·Douglas M SwankSanford I Bernstein
Dec 13, 2002·American Journal of Physiology. Cell Physiology·Kimberly Palmiter LittlefieldSanford I Bernstein
Sep 2, 2003·Molecular Biology and Evolution·Stephan SchmitzClaudia Veigel
Feb 7, 2004·The EMBO Journal·Bogos AgianianBelinda Bullard
Aug 10, 2004·Biophysical Journal·Marco LinariGabriella Piazzesi
Dec 8, 2004·The Journal of Experimental Biology·Michael S Tu, Thomas L Daniel
Jul 1, 2005·Physiological Reviews·Scott L Hooper, Jeffrey B Thuma
Jul 29, 2005·Molecular Biology and Evolution·Raúl HerranzRoberto Marco
Oct 19, 2005·Advances in Protein Chemistry·Michael A Geeves, Kenneth C Holmes
Feb 9, 2006·Journal of Muscle Research and Cell Motility·Agnes Ayme-SouthgateCatherine Wussler
Jun 6, 2006·Journal of Muscle Research and Cell Motility·Jesús MateosRoberto Marco
Sep 18, 2007·Journal of Molecular Biology·Sabrina E BoussoufMichael A Geeves
Jun 17, 2008·The Journal of Experimental Biology·Holly A Shiels, Ed White
Nov 5, 2008·Journal of Molecular Evolution·Agnes J Ayme-SouthgateCatherine Kramp
Apr 28, 2009·Journal of Molecular Biology·Marieke J BloeminkSanford I Bernstein
Jun 19, 2009·Cell Calcium·Yubin ZhouJenny J Yang
Jan 27, 2010·Journal of Molecular Biology·Uros KrzicBelinda Bullard
Oct 23, 2010·Archives of Biochemistry and Biophysics·Bin Wei, J-P Jin
Dec 15, 2010·Proceedings of the National Academy of Sciences of the United States of America·Robert J Perz-EdwardsMichael K Reedy
Feb 25, 2011·The Journal of Experimental Biology·Nicole T George, Tom L Daniel
Aug 1, 2002·Integrative and Comparative Biology·Douglas A Syme, Robert K Josephson
Nov 23, 2011·Journal of Muscle Research and Cell Motility·Belinda Bullard, Annalisa Pastore
Jan 8, 2013·Biochemical and Biophysical Research Communications·Hiroyuki Iwamoto
Nov 12, 2013·Journal of Insect Science·A J Ayme-SouthgateR J Southgate
Nov 26, 2013·Journal of Molecular Evolution·Felipe N Soto-AdamesJim O Vigoreaux
Jan 21, 2015·Archives of Biochemistry and Biophysics·Chen-Ching YuanThomas C Irving

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Citations

Aug 16, 2016·Insect Biochemistry and Molecular Biology·Michael R KanostGary W Blissard
Mar 14, 2018·Infection and Immunity·Luciana GalettoCristina Marzachì
Apr 28, 2020·Insect Molecular Biology·H-F LiangX-D Li

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