Myogenic differentiation triggered by antisense acidic fibroblast growth factor RNA.

Molecular and Cellular Biology
J C FoxJ L Swain

Abstract

Acidic fibroblast growth factor (FGF) and related family members regulate differentiation in organisms as diverse as Xenopus laevis and mammals. We utilized a well-characterized model of myogenic development to directly assess the importance of endogenously produced FGF in controlling differentiation. A role for endogenous FGF is suggested by the previous finding that acidic and basic FGF abundance in cultured myocytes decreases during differentiation. In this study we inhibited the endogenous production of FGF in murine Sol 8 myoblasts by using antisense RNA and observed precocious myogenic differentiation. Exogenously supplied acidic FGF rescues this phenotype. Further results suggest that the effect of FGF on myogenic differentiation is mediated in part through inhibition of myogenin expression. These results demonstrate a direct role for endogenously synthesized growth factors in regulating myogenesis and provide support for a general role for related proteins in mammalian development.

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Citations

Mar 6, 2009·Domestic Animal Endocrinology·R P RhoadsM V Dodson
Jul 2, 1999·Experimental Cell Research·K A ScataJ L Swain
Mar 4, 2014·PloS One·Somali ChaterjiAaron B Baker
Sep 11, 1998·Developmental Dynamics : an Official Publication of the American Association of Anatomists·A YamaneH C Slavkin
Dec 16, 1998·Developmental Dynamics : an Official Publication of the American Association of Anatomists·I LeconteJ L Swain
May 24, 1996·The Journal of Biological Chemistry·J C Fox, J R Shanley
Aug 1, 1996·Therapeutic Drug Monitoring·J C Fox
Sep 13, 2002·American Journal of Physiology. Cell Physiology·Yuji YoshikoNorihiko Maeda

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