PMID: 12788661Jun 6, 2003Paper

Myoglobin gene expression attenuates hepatic ischemia reperfusion injury

The Journal of Surgical Research
Takashi NittaYoshio Yamaoka

Abstract

Cellular functions are maintained by a continuous supply of ATP, which is supplied efficiently by mitochondrial oxidative phosphorylation. Since myoglobin, found in cardiac myocytes and red skeletal muscle, but not in the liver, facilitates oxygen diffusion under low oxygen conditions and enhances oxidative phosphorylation, this study seeks to enhance hepatic ATP levels and attenuate ischemia-reperfusion injury in rodent livers by adenovirus-mediated myoglobin expression. After infecting Hep3B and rodent livers with adenovirus carrying CMV promoter sequences linked to the human myoglobin gene (AdCMVMyo), reverse transcriptase-PCR and immunodetection for myoglobin, and cellular and hepatic ATP levels were examined. The effect of myoglobin was evaluated in a hepatic ischemia-reperfusion model in the rat. Myoglobin expression was confirmed in Hep3B and rat livers after AdCMVMyo infection. The ATP levels in Hep3B cells and C57BL/6 mice livers 72 h after AdCMVMyo transfection were significantly higher than control levels and those after adenovirus-mediated beta-galactosidase transfection. Finally, expression of myoglobin attenuated ischemia-reperfusion injury in the rat liver. These results indicate that myoglobin gene transfer to t...Continue Reading

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Citations

Dec 15, 2007·Veterinary Research Communications·Chuang Xu, Zhe Wang
Nov 14, 2006·Proceedings of the National Academy of Sciences of the United States of America·Adil A KhanDavid A Greenberg
Feb 14, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jane FraserAndrew R Cossins
Apr 12, 2016·The American Journal of Emergency Medicine·Liqiong YaoYunlin Tian
Jun 10, 2010·British Journal of Cancer·G KristiansenE Dahl
Oct 28, 2019·Mammalian Genome : Official Journal of the International Mammalian Genome Society·H J Abu-Toamih AtamniFuad A Iraqi
Oct 6, 2006·Current Opinion in Anaesthesiology·Claus-Georg KrennHubert Hetz
Aug 3, 2010·The Journal of Experimental Biology·U B Hendgen-CottaT Rassaf

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