Myoglobin intron variation in the Gouldian Finch Erythrura gouldiae assessed by temperature gradient gel electrophoresis

Electrophoresis
M M HeslewoodP R Baverstock

Abstract

In this study we show how the use of exon-primed, intron-crossing (EPIC) polymerase chain reaction (PCR) of a diploid intronic region, in conjunction with temperature gradient gel electrophoresis (TGGE), can be used to detect and rapidly assess allelic variation at the nucleotide level. We developed passerine-specific primers to amplify and sequence a 762 bp region including the second intron of the myoglobin gene in the Gouldian Finch, Erythrura gouldiae. A POLAND plot based on this sequence indicated that TGGE in combination with heteroduplex analysis (TGGE/HA) should reveal nucleotide variation in the 160 bp low-melting domain. Sequencing of the entire fragment from 19 Er. gouldiae revealed five nucleotide substitution differences within the low-melt domain, all of which could be detected and differentiated by TGGE/HA, and an additional substitution in a section of the high-melt domain which characterised another allele. A total of 181 individuals from four populations were screened for these six alleles.

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Citations

Jul 9, 2002·Molecular Phylogenetics and Evolution·Martin IrestedtPer G P Ericson
Sep 12, 2003·Molecular Phylogenetics and Evolution·Per G P Ericson, Ulf S Johansson
Jul 24, 2003·BMC Evolutionary Biology·Per G P EricsonJanette A Norman
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May 1, 2007·Molecular Phylogenetics and Evolution·Eric PasquetVincent Bretagnolle
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Sep 1, 2004·Molecular Phylogenetics and Evolution·Jérôme FuchsEric Pasquet
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Jul 9, 2013·Molecular Phylogenetics and Evolution·Ulf S JohanssonPer G P Ericson
Oct 16, 2013·Molecular Phylogenetics and Evolution·Marie AggerbeckKnud Andreas Jønsson
Dec 1, 2017·Proceedings. Biological Sciences·Kirstin Janssen, Nicholas I Mundy
Nov 20, 2009·Evolution; International Journal of Organic Evolution·Sarah R Pryke
Nov 9, 2021·Molecular Phylogenetics and Evolution·Somayeh PourebrahimiMansour Aliabadian

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