Myoglobin structure and function: A multiweek biochemistry laboratory project

Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology
Todd P SilversteinKaren L McFarlane Holman

Abstract

We have developed a multiweek laboratory project in which students isolate myoglobin and characterize its structure, function, and redox state. The important laboratory techniques covered in this project include size-exclusion chromatography, electrophoresis, spectrophotometric titration, and FTIR spectroscopy. Regarding protein structure, students work with computer modeling and visualization of myoglobin and its homologues, after which they spectroscopically characterize its thermal denaturation. Students also study protein function (ligand binding equilibrium) and are instructed on topics in data analysis (calibration curves, nonlinear vs. linear regression). This upper division biochemistry laboratory project is a challenging and rewarding one that not only exposes students to a wide variety of important biochemical laboratory techniques but also ties those techniques together to work with a single readily available and easily characterized protein, myoglobin.

References

Jul 18, 2008·Proceedings of the National Academy of Sciences of the United States of America·Ulrike B Hendgen-CottaTienush Rassaf
Jul 25, 2008·Nature·Andrew Cossins, Michael Berenbrink
Jun 15, 2011·Journal of Chemical Information and Modeling·Kalidas Yeturu, Nagasuma Chandra
Aug 22, 2012·Epidemiology and Infection·C K PorterM S Riddle

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Citations

Dec 8, 2015·Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology·Urmi Roy
Mar 26, 2021·Clinical Medicine : Journal of the Royal College of Physicians of London·Abdulrahman Al-NaseemJecko Thachil
Sep 7, 2021·Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology·Ohad Levkovich, Anat Yarden
Oct 4, 2021·Protein Science : a Publication of the Protein Society·Christine ZardeckiStephen K Burley

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