Sep 1, 1978

Myoinositol and phosphatidylinositol metabolism in synaptosomes from galactose-fed rats

Proceedings of the National Academy of Sciences of the United States of America
A S Warfield, S Segal

Abstract

The effects of experimental galactose toxicity on inositol and phosphatidylinositol (PtdIns) metabolism in synaptosomes from 0- to 30-day-old rats were investigated. Galactose toxicity was induced by feeding mothers a 40% galactose diet from the 12th day of pregnancy until 19 days postpartum when the offspring were weaned onto the maternal diet. There was no decrease in myoinositol concentrations and only a small decrease in PtdIns in synaptosomes from galactose-fed rats relative to glucose-fed controls. Synaptosomes from rats on the two diets converted equivalent amounts of [U-14C]glucose to inositol and PtdIns. Acetylcholine stimulated [2-3H]inositol incorporation into PtdIns while producing a net decrease in PtdIns concentration in synaptosomes from 22- to 30-day-old rats. However, the phospholipid response to acetylcholine in synaptosomes from galactose-fed rats was impaired. Thus, the acetylcholine-stimulated labeling of PtdIns was 40--50% lower in these synaptosomes while the effect on PtdIns concentration was reduced by a maximum of 55%. The data suggest that galactose-fed rats may have either a deficiency in the number of acetylcholine receptors or a defect in some step between receptor-neurotransmitter interaction and ...Continue Reading

Mentioned in this Paper

Metabolic Process, Cellular
Phosphatidylinositols
Galactose Measurement
Diet
Myasthenic Syndrome, Congenital, Associated With Acetylcholine Receptor Deficiency
Brain
Thyroid Hormone Plasma Membrane Transport Defect
Glucose, (beta-D)-Isomer
Cholinergic Receptors
Galactose

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