Myoinositol CEST signal in animals with increased Iba-1 levels in response to an inflammatory challenge-Preliminary findings

PloS One
Maria Yanez LopezHenryk M Faas

Abstract

Neuroinflammation plays an important role in the pathogenesis of a range of brain disorders. Non-invasive imaging of neuroinflammation is critical to help improve our understanding of the underlying disease mechanisms, monitor therapies and guide drug development. Generally, MRI lacks specificity to molecular imaging biomarkers, but molecular MR imaging based on chemical exchange saturation transfer (CEST) can potentially detect changes of myoinositol, a putative glial marker that may index neuroinflammation. In this pilot study we aimed to investigate, through validation with immunohistochemistry and in vivo magnetic resonance spectroscopy (MRS), whether CEST imaging can reflect the microglial response to a mild inflammatory challenge with lipopolysaccharide (LPS), in the APPSwe/ PS1 mouse model of Alzheimer's disease and wild type controls. The response to the immune challenge was variable and did not align with genotype. Animals with a strong response to LPS (Iba1+, n = 6) showed an increase in CEST contrast compared with those who did not (Iba1-, n = 6). Changes of myoinositol levels after LPS were not significant. We discuss the difficulties of this mild inflammatory model, the role of myoinositol as a glial biomarker, and...Continue Reading

References

Dec 1, 1986·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·J HennigH Friedburg
Jan 1, 1993·Developmental Neuroscience·A BrandD Leibfritz
Dec 1, 1993·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·S W Provencher
Jun 1, 1993·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·R M HenkelmanM J Bronskill
Jun 1, 1993·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·R Gruetter
May 20, 1999·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·I TkácR Gruetter
Oct 25, 2001·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·J G Sled, G B Pike
Dec 13, 2001·Journal of Magnetic Resonance·M Garwood, L DelaBarre
Jan 5, 2002·Neurobiology of Aging·G DiCarloD Morgan
Mar 31, 2005·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Donald E WoessnerA Dean Sherry
Oct 7, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Colm CunninghamV Hugh Perry
May 15, 2008·Journal of Neuroinflammation·Christopher J HenryJonathan P Godbout
Mar 24, 2009·Annual Review of Immunology·Richard M Ransohoff, V Hugh Perry
Apr 10, 2009·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Mina KimPeter C M van Zijl
Sep 9, 2009·Brain, Behavior, and Immunity·William A Banks, Sandra M Robinson
Dec 2, 2009·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Kristine M RobinsonRichard E Jones
Mar 10, 2010·NeuroImage·Joanna M AdamczakMathias Hoehn
Oct 19, 2010·NeuroImage·Mohammad HarisRavinder Reddy
Oct 11, 2011·Brain, Behavior, and Immunity·Peggy SchneiderCornelius R Pawlak
Oct 10, 2012·NMR in Biomedicine·Mohammad HarisRavinder Reddy
Jan 12, 2013·Science·Adriano AguzziMariko L Bennett
Jan 17, 2013·Proceedings of the National Academy of Sciences of the United States of America·Jean-Philippe MichaudSerge Rivest
May 15, 2013·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Linda ChangThomas Ernst
Oct 15, 2013·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Guanshu LiuPeter C M van Zijl
Oct 28, 2014·Brain Pathology·Natalie M ZahrAdolf Pfefferbaum
Dec 5, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Melissa E MurrayKejal Kantarci
Mar 17, 2015·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Derya Gol Gungor, Lee C Potter
Mar 21, 2015·Lancet Neurology·Michael T HenekaMarkus P Kummer
Mar 31, 2015·Nature Communications·Xiaolei SongJeff W M Bulte
Apr 3, 2015·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Maria-Angeles Carrillo-de SauvageCarole Escartin
Jul 15, 2015·Contrast Media & Molecular Imaging·Jae-Seung LeeRavinder R Regatte
Sep 12, 2015·Seminars in Vascular Surgery·Ramesh K Tripathi
Sep 29, 2015·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Daniel James ClarkSeth A Smith
Nov 10, 2015·Biochemical Society Transactions·Federico E TurkheimerMattia Veronese
Mar 18, 2016·ACS Chemical Neuroscience·Daniel S AlbrechtMarco L Loggia

❮ Previous
Next ❯

Citations

Feb 10, 2021·Biomedicines·Meredith A JonesLacey R McNally
Jun 21, 2021·Brain : a Journal of Neurology·Miriam RiesMagdalena Sastre

❮ Previous
Next ❯

Methods Mentioned

BETA
chemical exchange saturation transfer
NMR
transgenics
transgenic
imaging techniques

Software Mentioned

LCModel
Matlab
R
ARRIVE
Vnmrj

Related Concepts

Related Feeds

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here

Alzheimer's Disease: Neuroimaging

Neuroimaging can help identify pathological hallmarks of Alzheimer's disease (AD). Here is the latest research on neuroimaging modalities, including magnetic resonance imaging and positron emission tomography, in AD.

Alzheimer's Disease: Animal Models

Alzheimer's disease is a neurodegenerative disease which can be studied using various experimental systems. This feed focuses on animal models used for Alzheimer's disease research.

Alzheimer's Disease: Microglia

Microglia are a type of glial cell found throughout the brain and spinal cord. Microglia have been found to be associated with Alzheimer's disease development and progression. Here are the latest discoveries pertaining to Alzheimer's disease and microglia.