Myorelaxant activity of 2-t-butyl-4-methoxyphenol (BHA) in guinea pig gastric fundus

European Journal of Pharmacology
F FusiG Sgaragli


This study investigates the mechanism whereby the antioxidant 2-t-butyl-4-methoxyphenol (BHA) relaxes guinea pig gastric fundus smooth muscle. In circular smooth muscle strips, 10 microM cyclopiazonic acid, a specific inhibitor of sarcoplasmic reticulum Ca2+-ATPase, induced a prolonged rise in tension which depended on the presence of extracellular Ca2+. BHA (pIC50 = 5.83), sodium nitroprusside (6.85), isoproterenol (7.69) and nifedipine (8.02), but not 2,6-di-t-butyl-4-methoxyphenol (DTBHA) (up to 30 microM), relaxed muscle strips contracted with cyclopiazonic acid. Methyl-1,4-dihydro-2,6-dimethyl-3-nitro-4-(2-trifluoromethylphenyl)-pyri dine-5-carboxylate (Bay K 8644) (1 microM) antagonised the nifedipine- but not the BHA-induced relaxation. Nifedipine and isoproterenol (10 microM) caused a decrease in spontaneous tone, but did not counteract the subsequent rise in tension elicited by 10 microM cyclopiazonic acid. Conversely, 100 microM BHA and 100 microM sodium nitroprusside not only significantly reduced spontaneous tone but also markedly impaired the response of the muscles to cyclopiazonic acid. DTBHA failed to show either effect. When added to preparations completely relaxed by 100 microM BHA, 10 mM tetraethylammonium st...Continue Reading


Nov 11, 1990·Cell Calcium·J W Putney
Jul 31, 1987·Biochemical and Biophysical Research Communications·F ZoccaratoA Alexandre
Apr 15, 1988·Biochemical and Biophysical Research Communications·M IinoM Endo
Jan 1, 1974·Reviews of Physiology, Biochemistry and Pharmacology·M P Blaustein
Nov 15, 1995·The Biochemical Journal·M J Berridge
Sep 1, 1993·British Journal of Pharmacology·G P SgaragliP Mantovani
Mar 1, 1995·Trends in Pharmacological Sciences·C van BreemenI Laher
Nov 17, 1994·Nature·A P Somlyo, A V Somlyo
Mar 1, 1994·Trends in Pharmacological Sciences·C FasolatoT Pozzan
Sep 1, 1993·Trends in Pharmacological Sciences·U Quast
Apr 1, 1993·Journal of Muscle Research and Cell Motility·L Raeymaekers, F Wuytack

❮ Previous
Next ❯


Apr 20, 2000·European Journal of Pharmacology·F FusiG Sgaragli
Mar 17, 1999·European Journal of Pharmacology·G V Petkov, K K Boev
Mar 19, 1999·European Journal of Pharmacology·F FusiG P Sgaragli

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antihypertensive Agents: Mechanisms of Action

Antihypertensive drugs are used to treat hypertension (high blood pressure) which aims to prevent the complications of high blood pressure, such as stroke and myocardial infarction. Discover the latest research on antihypertensive drugs and their mechanism of action here.