MyoSight-semi-automated image analysis of skeletal muscle cross sections.

Skeletal Muscle
Lyle W BabcockSusan L Hamilton

Abstract

Manual analysis of cross-sectional area, fiber-type distribution, and total and centralized nuclei in skeletal muscle cross sections is tedious and time consuming, necessitating an accurate, automated method of analysis. While several excellent programs are available, our analyses of skeletal muscle disease models suggest the need for additional features and flexibility to adequately describe disease pathology. We introduce a new semi-automated analysis program, MyoSight, which is designed to facilitate image analysis of skeletal muscle cross sections and provide additional flexibility in the analyses. We describe staining and imaging methods that generate high-quality images of immunofluorescent-labelled cross sections from mouse skeletal muscle. Using these methods, we can analyze up to 5 different fluorophores in a single image, allowing simultaneous analyses of perinuclei, central nuclei, fiber size, and fiber-type distribution. MyoSight displays high reproducibility among users, and the data generated are in close agreement with data obtained from manual analyses of cross-sectional area (CSA), fiber number, fiber-type distribution, and number and localization of myonuclei. Furthermore, MyoSight clearly delineates changes i...Continue Reading

References

Jan 11, 2007·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Yoo-Jin KimJoachim Weickert
Sep 15, 2010·Annals of Neurology·J M WilmshurstHeinz Jungbluth
Jan 1, 2014·Skeletal Muscle·Lucas R Smith, Elisabeth R Barton
May 26, 2015·Computers in Biology and Medicine·Mauro MiazakiXiaoyin Xu
Oct 7, 2017·Journal of Applied Physiology·Yuan WenKenneth S Campbell
Aug 8, 2018·Skeletal Muscle·Alicia Mayeuf-LouchartAnne Danckaert
Aug 15, 2018·Journal of Neuromuscular Diseases·Hichem TasfaoutJocelyn Laporte
Oct 20, 2018·Skeletal Muscle·Yeh Siang LauRenzhi Han
Oct 4, 2019·Developmental Medicine and Child Neurology·Kristl G Claeys

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Citations

Apr 1, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Amy D HannaSusan L Hamilton

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Methods Mentioned

BETA
confocal microscopy

Software Mentioned

CSAM
Muscle J
ZEN Black
MyoSight
- CSAM
BioFormat
FIJI
Windows
Format
SMASH

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