Myosin cooperates with microtubule motors during organelle transport in melanophores

Current Biology : CB
S L Rogers, V I Gelfand

Abstract

Melanophores offer an outstanding system for the study of intracellular motility. These cells aggregate their pigment-filled melanosomes to the cell center or disperse them throughout the cytoplasm in response to hormonal modulation of intracellular cyclic AMP levels in order to effect color changes in lower vertebrates [1]. Previous work from our laboratory demonstrated a role for microtubule-based motors in melanosome transport and we succeeded in reconstituting their regulated motility along microtubules in vitro [2,3]. Here we demonstrate that, in addition to microtubule-mediated motility, melanosomes purified from Xenopus melanophores exhibit unidirectional movement along actin filaments in vitro as well. Immunoblotting analysis shows that these organelles possess the actin-based organelle motor, myosin-V. In vivo, melanosomes are able to slowly disperse in the absence of microtubules, and this slow dispersion requires the integrity of the actin cytoskeleton. Furthermore, in cells with dispersed pigment, disruption of filamentous actin induces a rapid, microtubule-dependent aggregation of melanosomes to the cell center. Our results, together with the accompanying paper by Rodionov et al. [4], demonstrate that the concerted...Continue Reading

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Citations

Jul 13, 2000·Cell Motility and the Cytoskeleton·E Frixione
Sep 26, 2000·Cell Motility and the Cytoskeleton·M KimbleE L de Hostos
Mar 29, 2002·Cell Motility and the Cytoskeleton·João Carlos da Silva BizarioEnilza Maria Espreafico
Jun 14, 2003·Medical & Biological Engineering & Computing·C ImmerstrandK H Peterson
Jul 14, 1999·Cell Biochemistry and Biophysics·T SoldatiE C Schwarz
Nov 5, 2003·Advanced Drug Delivery Reviews·Judy A Garner
Nov 5, 2003·Advanced Drug Delivery Reviews·John W Murray, Allan W Wolkoff
Jun 16, 2004·Current Opinion in Cell Biology·Deborah J FrankKathryn G Miller
Aug 28, 2003·Experimental Cell Research·Maria T Damiani, Maria I Colombo
Jun 6, 2003·Advanced Drug Delivery Reviews·Edward M Campbell, Thomas J Hope
Mar 21, 2001·Biochimica Et Biophysica Acta·S MaR L Chisholm
Feb 19, 2000·Current Opinion in Cell Biology·X WuJ A Hammer
Feb 19, 2000·Current Opinion in Cell Biology·S L Rogers, V I Gelfand
Feb 19, 2000·Current Opinion in Cell Biology·B L GoodeG Barnes
Feb 27, 1999·Current Opinion in Cell Biology·V Moreau, M Way
Aug 2, 2005·Nature Cell Biology·Ann L Miller, William M Bement
Apr 12, 2006·Nature Reviews. Molecular Cell Biology·Lois S Weisman
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Sep 26, 2013·Proceedings of the National Academy of Sciences of the United States of America·Susumu AoyamaYuichi Hiratsuka
Apr 10, 1999·Molecular Biology of the Cell·S L Reck-PetersonM S Mooseker
May 8, 1999·Molecular Biology of the Cell·G RaposoE Coudrier
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Apr 29, 2005·Molecular Biology of the Cell·Daniela A BritoMichael P Koonce
Feb 11, 2011·Molecular Biology of the Cell·Kazuho IkedaVladimir Rodionov
Nov 30, 1999·Pigment Cell Research·M C Tuma, V I Gelfand
Jun 12, 2013·Annual Review of Cell and Developmental Biology·Jonathan M Scholey
Dec 28, 1999·Annual Review of Cell and Developmental Biology·S S Brown

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