PMID: 8607995Apr 1, 1996Paper

Myosin drives retrograde F-actin flow in neuronal growth cones

Neuron
C H LinP Forscher

Abstract

Actin filaments assembled at the leading edge of neuronal growth cones are centripetally transported via retrograde F-actin flow, a process fundamental to growth cone guidance and other forms of directed cell motility. Here we investigated the role of myosins in retrograde flow, using two distinct modes of myosin inhibition: microinjection of NEM inactivated myosin S1 fragments, or treatment with 2,3-butanedione-2-monoxime, and inhibitor of myosin ATPase. Both treatments resulted in dose-dependent attenuation of retrograde F-actin flow and growth of filopodia. Growth was cytochalasin sensitive and directly proportional to the degree of myosin inhibition, suggesting that retrograde flow results from superimposition of two independent processes: actin assembly and myosin-based filament retraction. These results provide the first direct evidence for myosin involvement in neuronal growth cone function.

References

Jul 1, 1979·The Journal of Cell Biology·R L Meeusen, W Z Cande
Feb 1, 1992·Current Opinion in Neurobiology·M Tessier-Lavigne
Jan 1, 1991·Cell Motility and the Cytoskeleton·J P Heath, B F Holifield
Jul 11, 1991·Nature·J A Theriot, T J Mitchison
Apr 1, 1988·Journal of Muscle Research and Cell Motility·K HoriutiS Kurihara
Jul 1, 1989·The Journal of Physiology·N R AlpertL A Mulieri
Nov 1, 1988·Neuron·T Mitchison, M Kirschner
Jan 1, 1988·Cell Motility and the Cytoskeleton·G W FisherD L Taylor
Feb 19, 1988·Science·D Bray, J G White
Jan 1, 1986·Annual Review of Biochemistry·T D Pollard, J A Cooper
Apr 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·D Bray
Jan 1, 1982·Methods in Enzymology·S S Margossian, S Lowey
Oct 1, 1995·The Journal of Cell Biology·L P Cramer, T J Mitchison
Feb 1, 1995·Current Opinion in Neurobiology·T E Kennedy, M Tessier-Lavigne
Dec 1, 1994·The Journal of Cell Biology·J LeeK Jacobson
Oct 1, 1994·Current Opinion in Neurobiology·C H LinP Forscher
Jan 1, 1994·Annual Review of Biophysics and Biomolecular Structure·K Svoboda, S M Block
Feb 1, 1994·Current Opinion in Cell Biology·L P CramerJ A Theriot
Mar 1, 1993·The Journal of Cell Biology·C RuppertM Bähler
Mar 11, 1993·Nature·J LeeK Jacobson
Jun 1, 1993·The Journal of Cell Biology·C H Lin, P Forscher
Jan 1, 1995·Annual Review of Cell and Developmental Biology·M S Mooseker, R E Cheney

❮ Previous
Next ❯

Citations

Dec 19, 1998·Microscopy Research and Technique·M BaillyJ E Segall
Jan 29, 2000·Microscopy Research and Technique·A Buchstaller, D G Jay
Jan 1, 1997·Cell Motility and the Cytoskeleton·E A WelnhoferC S Cohan
Mar 21, 2000·Journal of Neuroscience Research·A RenaudinL McKerracher
Feb 19, 1998·Journal of Neurobiology·V Rehder, S Cheng
Nov 28, 2000·Cell Motility and the Cytoskeleton·S ChengV Rehder
Aug 10, 2000·Journal of Neurobiology·G Gallo, P C Letourneau
Jul 12, 2002·Cell Motility and the Cytoskeleton·Paul C Bridgman
Aug 26, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·W N LianC H Lin
Mar 14, 2002·Journal of Neurobiology·Su ChengVincent Rehder
Sep 5, 2002·Journal of Neurobiology·John T SchmidtByunghee Leu
Mar 21, 2001·Journal of Neurobiology·Y S KimM F VanBerkum
May 7, 2013·European Biophysics Journal : EBJ·Philipp RauchJosef A Käs
Dec 21, 2004·Journal of Molecular Histology·Marita Goodwin, Alpha S Yap
Aug 1, 1992·Trends in Cell Biology·J A Theriot, T J Mitchison
Jun 21, 2005·Medical Engineering & Physics·J Bereiter-Hahn
Feb 13, 2001·Current Opinion in Cell Biology·T Hasson, R E Cheney
Feb 1, 1997·Current Opinion in Cell Biology·M D WelchT J Mitchison
Nov 18, 1998·Current Opinion in Cell Biology·C G Galbraith, M P Sheetz
Nov 18, 1998·Current Opinion in Cell Biology·C L Adams, W J Nelson
Feb 27, 1999·Current Opinion in Cell Biology·C M Waterman-Storer, E Salmon
Feb 17, 2001·Current Opinion in Neurobiology·B J Dickson
Feb 7, 1998·Current Opinion in Neurobiology·T Hasson, M S Mooseker
Feb 1, 1997·Current Opinion in Neurobiology·L LuoY N Jan
May 6, 1998·Current Opinion in Neurobiology·D M Suter, P Forscher
Feb 23, 2002·Trends in Cell Biology·J Victor SmallKlemens Rottner
Feb 28, 2006·Nature Cell Biology·Nelson A MedeirosPaul Forscher
Jan 3, 2003·Nature Cell Biology·Xiao-bing YuanShumin Duan
Apr 19, 2005·Nature Neuroscience·Jorge Santos Da SilvaJose Abad-Rodriguez
Nov 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·Michael B SteketeeJeffrey L Goldberg
Aug 5, 2011·Proceedings of the National Academy of Sciences of the United States of America·Timo BetzJosef A Käs
Dec 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Dong WangMu-ming Poo
Nov 17, 1999·Molecular Biology of the Cell·K BurtonD L Taylor

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brain developing: Influences & Outcomes

This feed focuses on influences that affect the developing brain including genetics, fetal development, prenatal care, and gene-environment interactions. Here is the latest research in this field.