Myosin heavy chain turnover in cultured neonatal rat heart cells: effects of [Ca2+]i and contractile activity

The American Journal of Physiology
K L ByronAllen M Samarel

Abstract

Blockade of L-type Ca2+ channels in spontaneously contracting cultured neonatal rat ventricular myocytes causes contractile arrest, myofibrillar disassembly, and accelerated myofibrillar protein turnover. To determine whether myofibrillar protein turnover. To determine whether myofibrillar atrophy results indirectly from loss of mechanical signals or directly from alterations in intracellular Ca2+ concentration ([Ca2+]i), contractile activity was inhibited with verapamil (10 microM) or 2,3-butanedione monoxime (BDM), and their effects on cell shortening, [Ca2+]i, and myosin heavy chain (MHC) turnover were assessed. Control cells demonstrated spontaneous [Ca2+]i transients (peak amplitude 232 +/- 15 nM, 1-2 Hz) and vigorous contractile activity. Verapamil inhibited shortening by eliminating spontaneous [Ca2+]i transients. Low concentrations of BDM (5.0-7.5 mM) had no effect on basal or peak [Ca2+]i transient amplitude but reduced cell shortening, whereas 10 mM BDM reduced both [Ca2+]i transient amplitude and shortening. Both agents inhibited MHC synthesis, but only verapamil accelerated MHC degradation. Thus MHC half-life does not change in parallel with contractile activity but rather more closely follows changes in [Ca2+]i. [C...Continue Reading

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Citations

Mar 4, 2000·Circulation Research·S MiyataL A Leinwand
Jan 25, 2000·American Journal of Physiology. Heart and Circulatory Physiology·D J ChurchU Lang
Apr 25, 2000·American Journal of Physiology. Heart and Circulatory Physiology·D M EbleA M Samarel
Mar 10, 2000·Journal of Applied Physiology·B RussellW W Ashley
Jun 25, 1998·The American Journal of Physiology·A A Makhlouf, P J McDermott
Oct 29, 2004·Plant & Cell Physiology·Keisuke FunakiKeiichi Yamamoto
Jan 28, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Hongyan LiMichael B Ferrari
Mar 15, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Sophie TalonClaude Léoty
May 2, 2007·The Journal of General Physiology·Irina KulikovskayaSaul Winegrad
Jul 10, 2001·Acta Physiologica Scandinavica·J M ThomS K Hunter
Nov 30, 2010·Protoplasma·Janine E Radford, Rosemary G White
May 10, 2001·Journal of Molecular and Cellular Cardiology·A L BayerA M Samarel
Mar 14, 2002·Molecular Aspects of Medicine·G A Rutter

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