Myosin light chain kinase in endothelium: molecular cloning and regulation

American Journal of Respiratory Cell and Molecular Biology
J G GarciaA D Verin

Abstract

The phosphorylation of myosin light chains by myosin light chain kinase (MLCK) is a key event in agonist-mediated endothelial cell gap formation and vascular permeability. We now report the cloning and expression of a nonmuscle MLCK isoform in cultured endothelium. Screening of a human endothelial cell cDNA library identified a 7.7 kb cDNA with substantial (> 95%) homology to the coding region of the rabbit and bovine smooth muscle (SM) MLCK (amino acid #923-1913) as well as with the reported avian nonmuscle MLCK (65-70% homology). Sequence analysis also identified, however, a 5' stretch of novel sequence (amino acids #1-922) which is not contained in the open reading frame of mammalian SM MLCK, and is only 58% homologous to the avian fibroblast MLCK sequence. Immunoprecipitation with NH2-specific antisera revealed a 214 kD high molecular weight MLCK in bovine and human endothelium which exhibits MLC phosphorylation properties. Amino acid sequence analysis revealed endothelial MLCK consensus sequences for a variety of protein kinases including highly conserved potential phosphorylation sites for cAMP-dependent protein kinase A (PKA) in the CaM-binding region. Augmentation of intracellular cAMP levels markedly enhanced MLCK phos...Continue Reading

Citations

Oct 22, 2011·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Yoo Jeong HanJoe G N Garcia
Oct 6, 2007·Molecular Neurobiology·Evgeni PonimaskinAlexander Dityatev
Apr 27, 2004·Trends in Pharmacological Sciences·John H TinsleyEmily Wilson
Nov 1, 2002·Biochemical and Biophysical Research Communications·Steven M DudekJoe G N Garcia
Feb 27, 1999·Current Opinion in Cell Biology·A R Bresnick
Oct 3, 2008·Antioxidants & Redox Signaling·Srikanth PendyalaViswanathan Natarajan
Feb 4, 2009·Antioxidants & Redox Signaling·Konstantin G Birukov
Sep 3, 1998·Molecular Biology of the Cell·V Vouret-CraviariE Van Obberghen-Schilling
May 19, 2010·Cardiovascular Research·Qiang ShenSarah Y Yuan
Aug 20, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Janek HenesPeter Rosenberger
Jun 16, 2006·American Journal of Physiology. Cell Physiology·B Paul HerringJiliang Zhou
Feb 8, 2002·American Journal of Physiology. Cell Physiology·Emily K BluePatricia J Gallagher
Oct 4, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Cristhiaan D Ochoa, Troy Stevens
Dec 22, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·K L GrinnellE O Harrington
Oct 24, 2009·Journal of Applied Physiology·Anna A BirukovaKonstantin G Birukov
Nov 7, 1998·American Journal of Respiratory Cell and Molecular Biology·A D VerinJ G Garcia
Nov 7, 1998·American Journal of Respiratory Cell and Molecular Biology·A D VerinJ G Garcia
Jan 10, 2006·American Journal of Respiratory Cell and Molecular Biology·Li GaoJoe G N Garcia
Feb 9, 2010·American Journal of Respiratory Cell and Molecular Biology·Tamara MirzapoiazovaJoe G N Garcia
Mar 16, 2013·American Journal of Respiratory Cell and Molecular Biology·Djanybek M AdyshevJoe G N Garcia
May 6, 2011·Proceedings of the American Thoracic Society·Joe G N Garcia
Jan 17, 2014·American Journal of Respiratory Cell and Molecular Biology·Ting WangJoe G N Garcia
Jun 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jianjun BaoKazuhiro Kohama
Jan 12, 1999·Microvascular Research·L M DeFouw, D O DeFouw
Nov 4, 2000·The Journal of Cell Biology·A PoperechnayaA R Bresnick
Mar 17, 2007·Endothelium : Journal of Endothelial Cell Research·Fei HuangHazel Lum
Jun 26, 2002·The Journal of Cell Biology·Donna L CioffiTroy Stevens
Feb 4, 1999·Endothelium : Journal of Endothelial Cell Research·S ShiJ G Garcia
May 25, 2004·Journal of Cellular Biochemistry·Krisztina TarAlexander D Verin
Jun 7, 2011·Microvascular Research·Tracy Thennes, Dolly Mehta
Jan 27, 2007·The American Journal of Pathology·Hantamalala Ralay RanaivoRamaroson Andriantsitohaina
Aug 14, 2012·Medicinal Research Reviews·Robert R RigorSarah Y Yuan
Sep 8, 2004·Experimental Cell Research·Natalya G Dulyaninova, Anne R Bresnick

❮ Previous
Next ❯

Related Concepts

Related Feeds

American Thoracic Association Journals

Discover the latest respiratory research published by the journals from the American Thoracic Society.