Myosin light chain phosphatase and kinase abnormalities in fetal sheep pulmonary hypertension

Pediatric Research
J BelikM D Pato

Abstract

Inasmuch as smooth muscle contractile protein abnormalities may account for the maintenance of a high pulmonary vascular resistance, we evaluated the pulmonary arterial myosin light chain kinase (MLCK) and phosphatase (MLCP) in normal and pulmonary hypertensive (PH) fetal sheep. In addition, aorta and vena cava MLCP and MLCK activities were also measured. The MLCK activity (nanomoles/min/mg) was determined by the incorporation of [32P]PO4(-3) to the 20-kD smooth muscle myosin light chains and the MLCP activity by assaying for the dephosphorylation of the 20-kD myosin light chain (MLCP-light chain) and heavy meromyosin (MLCP-HMM). The MLCP content was determined by Western blot analysis. PH was characterized by a significant increase in the right-to-left ventricular wall weight ratio from 0.99 +/- 0.04 in the control to 1.52 +/- 0.12 in the experimental group (p < 0.01). The pulmonary MLCP-light chain and MLCP-HMM activities in the experimental group were 2.0 +/- 0.2 and 1.3 +/- 0.2 and significantly lower than in the control group values (3.8 +/- 0.5 and 2.5 +/- 0.3; p < 0.01). The MLCK activity was 9.6 +/- 1.2 in the control and 7.8 +/- 0.7 in the experimental fetal pulmonary artery (p = NS). The activities of both enzymes in ...Continue Reading

References

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Citations

Jun 26, 2020·Food Science and Technology International = Ciencia Y Tecnología De Los Alimentos Internacional·Namariq DhahirAmer AbuGhazaleh
Jun 11, 2002·American Journal of Physiology. Cell Physiology·Yongmu ZhengMichael E DiSanto
Oct 11, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·J BelikMary D Pato
Dec 8, 2005·Canadian Journal of Physiology and Pharmacology·Shyamala Dakshinamurti
Feb 3, 2012·Physiological Reviews·J T SylvesterJeremy P T Ward
Dec 8, 2005·Canadian Journal of Physiology and Pharmacology·Andrew J Halayko, Gerald L Stelmack
May 5, 2012·Pulmonary Circulation·Cassidy Delaney, David N Cornfield
Jun 8, 2001·Critical Care Medicine·J W BerkenboschT Perreault

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