Myosin light chain phosphorylation regulates barrier function by remodeling tight junction structure

Journal of Cell Science
Le ShenJ R Turner

Abstract

Epithelial tight junctions form a barrier against passive paracellular flux. This barrier is regulated by complex physiologic and pathophysiologic signals that acutely fine-tune tight junction permeability. Although actomyosin contraction and myosin light chain phosphorylation are clearly involved in some forms of tight junction regulation, the contributions of other signaling events and the role of myosin light chain phosphorylation in this response are poorly understood. Here we ask if activation of myosin light chain kinase alone is sufficient to induce downstream tight junction regulation. We use a confluent polarized intestinal epithelial cell model system in which constitutively active myosin light chain kinase, tMLCK, is expressed using an inducible promoter. tMLCK expression increases myosin light chain phosphorylation, reorganizes perijunctional F-actin, and increases tight junction permeability. TJ proteins ZO-1 and occludin are markedly redistributed, morphologically and biochemically, but effects on claudin-1 and claudin-2 are limited. tMLCK inhibition prevents changes in barrier function and tight junction organization induced by tMLCK expression, suggesting that these events both require myosin light chain phospho...Continue Reading

References

Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M Gossen, H Bujard
Jan 1, 1987·The Journal of Membrane Biology·J L Madara, J R Pappenheimer
Nov 7, 1995·Proceedings of the National Academy of Sciences of the United States of America·A NusratJ L Madara
Nov 11, 1996·The American Journal of Physiology·G HechtP D Lanerolle
Jul 14, 1998·The Journal of Cell Biology·T S JouW J Nelson
Sep 9, 1998·The American Journal of Physiology·S GopalakrishnanJ A Marrs
Apr 13, 1999·The American Journal of Physiology·T Y MaN Hoa
Nov 18, 2000·American Journal of Physiology. Cell Physiology·J R TurnerJ M Angle
Apr 5, 2001·American Journal of Physiology. Cell Physiology·Y YuA P Majumdar
May 2, 2001·The Journal of Cell Biology·K KatohK Fujiwara
Oct 16, 2001·American Journal of Physiology. Cell Physiology·J R Turner, E D Black
Nov 14, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·J J BerglundJ R Turner
Jun 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Heather A EdensCharles A Parkos
Nov 15, 2002·Journal of Cell Science·Salah AmashehMichael Fromm

❮ Previous
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Citations

Jul 16, 2008·Inflammatory Bowel Diseases·Michael A McGuckinGraham Radford-Smith
Sep 25, 2010·Journal of Neural Transmission·H C BauerH Bauer
Dec 29, 2007·Pharmaceutical Research·Linda M FeigheryDavid J Brayden
Nov 21, 2012·Nature Reviews. Gastroenterology & Hepatology·Michel NeunlistMalvyne Rolli-Derkinderen
Oct 27, 2009·Nature Reviews. Immunology·Jerrold R Turner
Apr 21, 2010·Proceedings of the National Academy of Sciences of the United States of America·Dan YuJerrold R Turner
Apr 19, 2012·Proceedings of the National Academy of Sciences of the United States of America·Karen L EdelblumJerrold R Turner
Jun 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Masahiko ItohHiroyuki Sugimoto
May 19, 2009·The Journal of Biological Chemistry·Georg LamprechtMichael Gregor
Oct 2, 2013·The Journal of Biological Chemistry·Minseon ParkMichal Toborek
Apr 9, 2010·Surgical Infections·Carrie Y PetersonRaul Coimbra
Jun 29, 2007·Molecular Biology of the Cell·Stanislav N SamarinAsma Nusrat
Oct 16, 2009·Molecular Biology of the Cell·Marouan AbouhamedMartin Bähler
Jul 17, 2009·Molecular Biology of the Cell·Christina M Van ItallieJames M Anderson
May 27, 2011·Molecular Biology of the Cell·Eitan E ZahaviBenjamin Aroeti
Mar 16, 2012·Molecular Biology of the Cell·Katy C LiuRichard E Cheney
May 11, 2012·Molecular Biology of the Cell·Surjit K Chandhoke, Mark S Mooseker
Jul 26, 2013·Molecular Biology of the Cell·Ana C MonteiroCharles A Parkos
Jul 23, 2009·Evidence-based Complementary and Alternative Medicine : ECAM·Aristo Vojdani, Jama Lambert
May 8, 2013·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jiali CaiZhenghong Zuo
Jan 12, 2007·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·András Kapus, Katalin Szászi
Mar 20, 2010·American Journal of Physiology. Cell Physiology·Faiza WaheedKatalin Szászi
Jul 14, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Anita ZahsElizabeth J Kovacs
Aug 2, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Ravinder K GillPradeep K Dudeja
Jan 31, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·Brian C DiPaolo, Susan S Margulies
Nov 10, 2009·Digestive Diseases·Le ShenJerrold R Turner
Apr 8, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Huabing QiFengjun Wang
Oct 4, 2006·The Journal of Clinical Investigation·Michael Field
May 9, 2012·The Journal of Clinical Investigation·Camille JungFrederick Barreau
Oct 26, 2011·Microbial Cell Factories·Jerry M Wells
Jun 23, 2012·PloS One·Taylor S CohenSusan S Margulies

❮ Previous
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