Myosin phenotype and bioenergetic characteristics of rat respiratory muscles

Medicine and Science in Sports and Exercise
Scott K PowersD Prezant

Abstract

These experiments examined the myosin phenotype and bioenergetic enzyme activities in rat respiratory muscles. Muscle samples were removed from adult female Sprague-Dawley rats (N = 8) and analyzed to determine the myosin heavy chain (MHC) and light chain (MLC) isoform content as well as the activities of myofibrillar ATPase (mATPase), citrate synthase (CS; Krebs cycle enzyme), and lactate dehydrogenase (LDH; glycolytic enzyme). Analysis revealed that CS activity and the % type I MHC and %IId MHC isoforms were greater in the costal diaphragm (CO-D) compared with those in the crural diaphragm (CR-D). In contrast, the % type IIb MHC was higher in the CR-D compared with that in the CO-D. LDH and mATPase activity were lower in both the CO-D and CR-D compared with that in the parasternal intercostals (PI), external intercostals (EI), internal intercostals (II), rectus abdominis (RA), and sternomastoid (SM) muscles. CS activity, % type I MHC, %IIa MHC, and the ratio of slow to total alkali MLC (1s/1s + 1f + 3f) were greater in the CO-D and CR-D compared with those in all other respiratory muscles. The RA contained the highest (P < 0.05) % type IIb MHC and lowest CS activity compared with that in all other muscles. Finally, CS activit...Continue Reading

References

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Citations

Sep 11, 2004·Journal of Applied Physiology·Tyrone A WashingtonJames A Carson
Dec 7, 2007·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Hua LinBinh Quach
Nov 24, 2004·Journal of Applied Physiology·Keith C DeRuisseauScott K Powers
Jul 12, 2013·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Scott K PowersAshley J Smuder
Apr 19, 2002·Journal of Applied Physiology·Scott K PowersStephen L Dodd
Oct 13, 2000·American Journal of Physiology. Cell Physiology·S K BortolottoG M Stephenson
May 6, 2006·Journal of Applied Physiology·D J FalkS K Powers
Jun 5, 2003·Journal of Applied Physiology·Murat A ZergerogluScott K Powers
Jan 20, 2019·Intensive Care Medicine Experimental·Vanessa ZambelliGiacomo Bellani
Aug 7, 2004·American Journal of Respiratory and Critical Care Medicine·R Andrew ShanelyScott K Powers
Feb 8, 2019·Journal of Applied Physiology·Tetsunori IkegamiPaul A Easton
Mar 2, 2012·Acta Cirúrgica Brasileira·André de Sá Braga OliveiraPaloma Lys de Medeiros
Mar 21, 2021·Clinical and Translational Science·Toshinori YoshiharaScott K Powers

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